Description

Gal1-B-4Glcnac-B-Pnp is a specialized glycosyltransferase substrate and a chromogenic synthetic compound used in enzymatic assays. This high-purity biochemical is critical for research and development in glycobiology and enzymology, enabling the study of glycosylation mechanisms. It is primarily utilized by pharmaceutical researchers, diagnostic kit manufacturers, and academic institutions focused on carbohydrate-active enzymes and biomarker discovery.

Application

• Glycosyltransferase Activity Assays: Serves as a key chromogenic substrate for measuring the activity of β-1,4-galactosyltransferases and related enzymes in vitro.
• Enzyme Kinetic Studies: Used to determine kinetic parameters (Km, Vmax) and inhibition constants for carbohydrate-processing enzymes.
• High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify potential inhibitors or modulators of glycosyltransferase activity.
• Diagnostic Development: Acts as a core reagent in the development of clinical diagnostic tests for conditions related to aberrant glycosylation.
• Biochemical Research: Facilitates fundamental research into the biosynthesis of complex carbohydrates, glycoproteins, and glycolipids.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of enzyme batches used in biopharmaceutical production processes.

Basic Information

Product Name	Gal1-B-4Glcnac-B-Pnp
CAS No.	74211-28-2
Molecular Formula	C24H32N2O13
Molecular Weight	556.52 g/mol
Synonyms	p-Nitrophenyl β-D-galactopyranosyl-(1→4)-2-acetamido-2-deoxy-β-D-glucopyranoside; Galβ1-4GlcNAcβ-PNP; N-Acetyllactosamine p-nitrophenyl glycoside; β-D-Gal-(1→4)-β-D-GlcNAc-OPhNO2; Lacto-N-biose I p-nitrophenyl derivative; PNP-β-LacNAc; Galβ1→4GlcNAcβ→O-PNP; 4-Nitrophenyl N-acetyl-β-lactosaminide
EINECS	Contact for details

Quality Control

Our Gal1-B-4Glcnac-B-Pnp is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. We adhere to relevant guidelines for research-grade biochemicals to support reproducible scientific results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO
Biological Activity	Suitable for glycosyltransferase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.