Description

n-(2-Nitro-4-Azidobenzoyl)Puromycin is a specialized biochemical reagent that functions as a photoaffinity labeling agent, incorporating a puromycin moiety with a photoreactive aryl azide group. This compound is critical for advanced proteomics research, enabling the covalent cross-linking and identification of biomolecular interaction sites upon UV light activation. It is primarily utilized by research scientists and pharmaceutical developers in the fields of molecular biology, biochemistry, and drug discovery for studying ribosome function, protein synthesis, and RNA-protein interactions.

Application

• Photoaffinity Labeling in Proteomics: Used to covalently tag and identify active sites in ribosomes, enzymes, and other macromolecular complexes.
• Mechanistic Studies of Translation: Employed as a probe to investigate the mechanisms of protein synthesis and ribosomal peptidyl transferase activity.
• Biomolecular Interaction Mapping: Essential for capturing transient or weak interactions between proteins, RNA, and small molecules in complex biological systems.
• Drug Discovery Target Validation: Serves as a tool for validating and characterizing potential drug targets involved in translation and cellular metabolism.
• Bioconjugation and Probe Development: Acts as a key intermediate for synthesizing more complex biochemical probes and affinity reagents.

Basic Information

Product Name	n-(2-Nitro-4-Azidobenzoyl)Puromycin
CAS No.	73579-23-4
Molecular Formula	C33H34N8O9
Molecular Weight	686.67 g/mol
Synonyms	2'-Deoxy-2'-(4-azido-3-nitrobenzamido)puromycin; N-[2-(4-Azido-3-nitrobenzoyl)amino]puromycin; Puromycin, N-[2-(4-azido-3-nitrobenzoyl)]-; Photoactivatable Puromycin; Azido Nitro Benzoyl Puromycin; ANB-Puromycin; Photoreactive Puromycin Analog
EINECS	Contact for details

Quality Control

Our n-(2-Nitro-4-Azidobenzoyl)Puromycin is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to relevant standards for high-purity biochemical reagents to support your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. Due to its strictly light-sensitive nature and potential thermal instability, the material should be handled under subdued light and allowed to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers
Functional Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.