Description

Boc-Glu(Obzl)-Gly-Arg-Mca Hydrochloride is a specialized fluorogenic peptide substrate designed for high-sensitivity protease activity assays. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of enzyme kinetics and inhibitor screening. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in protease-related drug discovery and biochemical analysis.

Application

• Protease Activity Assays: As a fluorogenic substrate for measuring the activity of various proteases, including trypsin, thrombin, and other serine proteases.
• Drug Discovery & Screening: High-throughput screening (HTS) of potential protease inhibitors for therapeutic development in areas like oncology, inflammation, and infectious diseases.
• Biochemical Research: Kinetic studies and characterization of enzyme mechanisms in academic and industrial research laboratories.
• Diagnostic Development: A key component in the development of sensitive diagnostic assays for detecting protease levels associated with specific pathological conditions.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of biopharmaceuticals to ensure product consistency and safety.

Basic Information

Product Name	Boc-Glu(Obzl)-Gly-Arg-Mca Hydrochloride
CAS No.	73554-94-6
Molecular Formula	C₃₇H₅₀N₈O₁₀ • HCl
Molecular Weight	811.31 g/mol (Free base)
Synonyms	Boc-Glu(OBzl)-Gly-Arg-MCA•HCl; Boc-Glu(OBzl)-Gly-Arg-AMC Hydrochloride; t-Butyloxycarbonyl-γ-benzyl-L-glutamyl-glycyl-L-arginine-4-methylcoumaryl-7-amide Hydrochloride; Boc-E(OBzl)-G-R-MCA HCl; Fluorogenic Substrate for Protease; Protease Substrate VII; Trypsin Substrate (Fluorogenic)
EINECS	Contact for details

Quality Control

Every batch of Boc-Glu(Obzl)-Gly-Arg-Mca Hydrochloride is manufactured and tested under strict quality management systems. We ensure high purity and batch-to-batch consistency through advanced analytical techniques including HPLC and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and other critical parameters are provided with each shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.