Description

Boc-Leu-Ser-Thr-Arg-Mca is a specialized fluorogenic peptide substrate designed for protease activity assays. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of enzymatic activity and inhibition. It is primarily utilized by researchers in academic institutions, pharmaceutical R&D laboratories, and biotechnology companies focused on protease-related drug discovery and biochemical pathway analysis.

Application

• Protease Activity Assays: As a fluorogenic substrate for measuring the activity of specific proteases, including trypsin and related serine proteases.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors for therapeutic development.
• Enzyme Kinetics Studies: Enables detailed kinetic analysis of protease enzymes, determining parameters such as Km and Vmax.
• Biochemical Research: A key tool for studying protease function, regulation, and role in biological processes like apoptosis and signal transduction.
• Diagnostic Development: Potential use in developing diagnostic assays for diseases where protease activity is a biomarker.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Boc-Leu-Ser-Thr-Arg-Mca
CAS No.	73554-93-5
Molecular Formula	C34H53N9O11
Molecular Weight	763.85 g/mol
Synonyms	Boc-Leu-Ser-Thr-Arg-7-amido-4-methylcoumarin; tert-Butyloxycarbonyl-Leucyl-Seryl-Threonyl-Arginyl-4-Methylcoumaryl-7-Amide; Boc-LSTR-MCA; Boc-Leu-Ser-Thr-Arg-AMC; Fluorogenic Substrate for Trypsin; Protease Substrate V; Trypsin Fluorogenic Substrate; LSTR-MCA
EINECS	Contact for details

Quality Control

Our Boc-Leu-Ser-Thr-Arg-Mca is produced under stringent quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.