Description

Boc-Phe-Ser-Arg-Amc Acetate Salt is a high-purity, fluorogenic peptide substrate designed for sensitive enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly thrombin and related enzymes. It is primarily utilized in pharmaceutical R&D, biochemical research, and diagnostic kit development for its reliable and quantifiable release of a fluorescent signal upon cleavage.

Application

• Protease Activity Assays: A premier fluorogenic substrate for the continuous kinetic measurement of thrombin and trypsin-like enzyme activity.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Development: Employed in the formulation of clinical diagnostic kits for monitoring blood coagulation pathways and related disorders.
• Biochemical Research: Serves as a key tool for studying enzyme kinetics, specificity, and mechanism of action in purified systems.
• Quality Control in Biologics: Applied in the purity and potency testing of therapeutic enzymes and related biopharmaceutical products.

Basic Information

Product Name	Boc-Phe-Ser-Arg-Amc Acetate Salt
CAS No.	73554-90-2
Molecular Formula	C₃₃H₅₃N₁₁O₁₀ • xC₂H₄O₂
Molecular Weight	~787.86 g/mol (free base)
Synonyms	Boc-Phe-Ser-Arg-AMC Acetate; t-Butyloxycarbonyl-Phenylalanyl-Seryl-Arginyl-7-Amino-4-Methylcoumarin Acetate; Boc-F-S-R-AMC Acetate; Thrombin Substrate I, Fluorogenic; Boc-Phe-Ser-Arg-AMC • AcOH; Benzyloxycarbonyl-Phe-Ser-Arg-AMC Acetate (common alternate); Fluorogenic Peptide Substrate for Thrombin; t-Boc-Phe-Ser-Arg-AMC Acetate Salt
EINECS	Contact for details

Quality Control

Our Boc-Phe-Ser-Arg-Amc Acetate Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.