Description

Boc-Glu-Lys-Lys-Mca CAS NO 73554-85-5 is a specialized, protected tetrapeptide derivative featuring a 7-methoxycoumarin-4-acetyl (Mca) fluorophore. This compound is of significant value as a key intermediate and substrate in biochemical research, particularly for the study of protease and peptidase enzyme activity. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers engaged in high-throughput screening and fluorescence-based assay development.

Application

• Fluorogenic Protease Substrate: Used as a sensitive substrate for monitoring the activity of various proteolytic enzymes, including cathepsins, caspases, and other serine or cysteine proteases.
• Biochemical Research & Assay Development: Serves as a critical reagent in developing and optimizing fluorescence resonance energy transfer (FRET) and other fluorescence-based enzymatic assays.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors for drug discovery programs.
• Diagnostic Reagent Component: Can be incorporated into diagnostic kits for detecting specific protease activities associated with disease states.
• Peptide Chemistry Intermediate: Acts as a building block in solid-phase peptide synthesis (SPPS) for constructing larger, more complex peptide sequences containing the Mca reporter group.

Basic Information

Product Name	Boc-Glu-Lys-Lys-Mca
CAS No.	73554-85-5
Molecular Formula	C41H62N8O13
Molecular Weight	875.0 g/mol (approx.)
Synonyms	Boc-Glu-Lys-Lys-MCA; t-Butyloxycarbonyl-Glutamyl-Lysyl-Lysyl-7-Methoxycoumarin-4-Acetyl; Boc-Glu(OtBu)-Lys(Boc)-Lys(Boc)-MCA; N-α-t-Butyloxycarbonyl-γ-t-butyl-L-glutamyl-N-ε-t-butyloxycarbonyl-L-lysyl-N-ε-t-butyloxycarbonyl-L-lysyl-7-methoxycoumarin-4-acetamide; Fluorogenic Peptide Substrate Mca-Glu-Lys-Lys; Protease Substrate V
EINECS	Contact for details

Quality Control

Every batch of Boc-Glu-Lys-Lys-Mca is subjected to rigorous analytical testing to ensure identity, purity, and performance consistency suitable for sensitive research applications. Our standard quality protocol includes HPLC for purity assessment, mass spectrometry (MS) for confirmation, and NMR for structural verification. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.