Description

Methylumbelliferyl 2-Acetamido-2-Deoxy-β-D-Lactoside CAS NO 73448-32-5 is a specialized fluorogenic substrate designed for the sensitive detection and quantification of β-N-acetylhexosaminidase activity. This compound is critical for researchers and quality control laboratories requiring precise enzymatic assays. It is primarily used in life science research, diagnostic kit manufacturing, and biochemical studies related to lysosomal storage disorders and carbohydrate metabolism.

Application

• Enzymatic Assay Substrate: A key fluorogenic substrate for the continuous spectrophotometric or fluorometric assay of β-N-acetylhexosaminidase (Hexosaminidase) activity.
• Diagnostic Development: Used in the research and production of diagnostic kits for conditions like Tay-Sachs and Sandhoff diseases, where hexosaminidase activity is a critical biomarker.
• Biochemical Research: Essential for studying enzyme kinetics, inhibitor screening, and characterizing glycosidase activity in various biological samples.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify potential therapeutic modulators of hexosaminidase activity.
• Microbiology: Used as a research tool to study bacterial glycosidases and their role in pathogenesis or carbohydrate processing.
• Quality Control: Serves as a reference standard in pharmaceutical and diagnostic manufacturing to ensure batch-to-batch consistency of enzyme-based products.

Basic Information

Item	Details
Product Name	Methylumbelliferyl 2-Acetamido-2-Deoxy-β-D-Lactoside
CAS No.	73448-32-5
Molecular Formula	C20H24N2O11
Molecular Weight	468.41 g/mol
Synonyms	4-Methylumbelliferyl N-acetyl-β-D-glucosaminide; 4-MU-β-GlcNAc; 4-Methylumbelliferyl 2-acetamido-2-deoxy-β-D-glucopyranoside; MUG-NAG; 4-MU-GlcNAc; β-N-Acetylglucosaminidase Substrate; Hexosaminidase Substrate; 4-Methylumbelliferyl β-N-acetylglucosaminide
EINECS	Contact for details

Quality Control

Every batch of Methylumbelliferyl 2-Acetamido-2-Deoxy-β-D-Lactoside is manufactured under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure compliance with high-purity research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and impurity profiles is provided with each shipment to guarantee traceability and performance consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Functional for enzyme assay
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.