Description

5-Iodo-Uridine-5'-Triphosphate, Sodium Salt is a modified nucleotide triphosphate where the 5-position of the uridine base is substituted with an iodine atom. This modification is critical for its primary role as a substrate for DNA polymerases in molecular biology techniques, particularly in next-generation sequencing (NGS) and labeling applications. It is an essential biochemical reagent for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic sectors requiring precise nucleic acid manipulation and detection.

Application

• Next-Generation Sequencing (NGS): Used as a reversible terminator in sequencing-by-synthesis platforms for high-throughput DNA analysis.
• DNA Labeling and Probe Synthesis: Serves as a substrate for incorporating iodine-labeled nucleotides into nucleic acid probes for hybridization assays and blotting techniques.
• Enzymatic Synthesis: Acts as a building block for the in vitro enzymatic synthesis of modified RNA or DNA strands.
• Biochemical Research: Utilized in studies of polymerase kinetics, fidelity, and mechanism due to its modified structure.
• Diagnostic Assay Development: Incorporated into nucleic acid-based tests for enhanced detection signals or specific labeling requirements.
• X-ray Crystallography: The heavy iodine atom can be used as a phasing tool in the structural determination of nucleic acid-protein complexes.

Basic Information

Product Name	5-Iodo-Uridine-5'-Triphosphate, Sodium Salt
CAS No.	73431-55-7
Molecular Formula	C9H11IN2Na2O14P3
Molecular Weight	688.97 g/mol (anhydrous basis)
Synonyms	5-Iodo-UTP, Sodium Salt; 5-Iodo-uridine-5'-triphosphate trisodium salt; 5-I-UTP; Iodo-UTP; 1-[(2R,3S,4R,5R)-5-(Hydroxy{hydroxy[hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl)-3,4-dihydroxyoxolan-2-yl]-5-iodopyrimidine-2,4-dione sodium salt; 5-Iodouridine-5'-(tetrahydrogen triphosphate), trisodium salt
EINECS	Contact for details

Quality Control

Our 5-Iodo-UTP, Sodium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for molecular biology-grade reagents to support your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0 %
Solubility	Soluble in water, slightly soluble in ethanol
Biological Activity	Suitable for polymerase incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.