Description

n-[4-[2-(2,5-Dioxo-4-Phenylimidazolidin-4-Yl)Phenoxy]Butyl]-7-(β-D-Galactopyranosyloxy)-2-Oxo-2H-1-Benzopyran-3-Carboxamide is a sophisticated synthetic organic compound designed for advanced biochemical and diagnostic applications. Its molecular architecture, featuring a coumarin fluorophore linked to a galactopyranosyl moiety and a hydantoin-derived spacer, makes it a highly specific enzymatic substrate and fluorescent probe. This compound is primarily utilized by research institutions and diagnostic manufacturers requiring precise, sensitive tools for enzyme activity detection and assay development.

Application

• Enzymatic Substrate for β-Galactosidase: A critical reagent in assays measuring β-galactosidase activity, commonly used in molecular biology (e.g., lacZ gene reporter systems) and diagnostic kits.
• Fluorescent Probe Development: Serves as a core building block for synthesizing advanced fluorescent probes and sensors targeting specific biological processes.
• Diagnostic Kit Manufacturing: Integrated into the production of clinical and research diagnostic kits for detecting enzymatic markers associated with various conditions.
• Biochemical Research: Used in high-throughput screening (HTS) and enzyme kinetics studies to investigate inhibitor efficacy and catalytic mechanisms.
• Cell-based Assays: Employed in live-cell imaging and flow cytometry to visualize and quantify β-galactosidase expression at the cellular level.
• Pharmaceutical R&D: Acts as a tool compound in drug discovery pipelines for validating targets related to glycosidase enzymes.

Basic Information

Product Name	n-[4-[2-(2,5-Dioxo-4-Phenylimidazolidin-4-Yl)Phenoxy]Butyl]-7-(β-D-Galactopyranosyloxy)-2-Oxo-2H-1-Benzopyran-3-Carboxamide
CAS No.	73304-29-7
Molecular Formula	C₃₅H₃₃N₃O₁₂
Molecular Weight	687.65 g/mol
Synonyms	7-(β-D-Galactopyranosyloxy)-2-oxo-2H-1-benzopyran-3-carboxylic acid 4-[2-(2,5-dioxo-4-phenyl-1-imidazolidinyl)phenoxy]butylamide; β-Galactosidase Substrate (Coumarin-derived); 4-MU-β-D-Galactopyranoside derivative; Fluorescent β-Gal substrate; Galactosyloxycoumarin carboxamide; Hydantoin-linked coumarin galactoside; Diagnostic probe 73304-29-7
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical is governed by a rigorous quality management system. Each batch is subjected to comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods (NMR, MS) for structural confirmation, to ensure consistency and performance. Certificates of Analysis (COA) detailing batch-specific results are provided and can be tailored to meet specific research or GMP-grade requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive; allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and exposure. Store in a cool, dry, and well-ventilated area.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines
Functional Activity (Enzymatic Assay)	≥ 90% of reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.