Description

H-Glu(Amc)-Oh CAS NO 72669-53-5 is a specialized fluorogenic peptide substrate, specifically L-Glutamic Acid γ-(7-Amino-4-methylcoumarin) Amide. This compound is a critical tool in biochemical research and diagnostic development, serving as a sensitive substrate for various proteolytic enzymes. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical, and diagnostic industries for enzyme activity assays and high-throughput screening applications.

Application

• Protease and Peptidase Assays: As a fluorogenic substrate for enzymes like glutamyl endopeptidases and other proteases that cleave at glutamic acid residues.
• Biochemical Research: For studying enzyme kinetics, inhibition, and mechanism of action in vitro.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify potential protease inhibitors or activators.
• Diagnostic Development: A key component in the development of fluorometric diagnostic kits for detecting specific enzymatic activity related to diseases.
• Cell Biology Studies: Employed in assays to monitor intracellular protease activity and related cellular processes.

Basic Information

Product Name	H-Glu(Amc)-Oh
CAS No.	72669-53-5
Molecular Formula	C15H17N3O5
Molecular Weight	319.31 g/mol
Synonyms	L-Glutamic Acid γ-(7-Amino-4-methylcoumarin) Amide; Glutamic Acid 7-Amido-4-methylcoumarin; Glu-AMC; L-Glu-AMC; H-Glu-AMC-OH; AMC-Glu; Substrate for Glutamyl Endopeptidase; Fluorogenic Glutamate Substrate; 7-Amino-4-methylcoumarin conjugate of L-glutamic acid
EINECS	Contact for details

Quality Control

Our H-Glu(Amc)-Oh is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.