Description

Recombinant Lysyl Endopeptidase is a highly specific serine protease that cleaves peptide bonds at the carboxyl side of lysine residues. This precision makes it an indispensable tool for protein sequencing, peptide mapping, and structural analysis in life science research and biopharmaceutical development. It is essential for researchers and manufacturers in proteomics, antibody-drug conjugate (ADC) characterization, and recombinant protein production who require exceptional specificity and activity to ensure accurate and reproducible results.

Application

• Proteomic Research & Protein Sequencing: Used for specific digestion of proteins prior to mass spectrometry (MS) analysis, enabling precise peptide mapping and identification.
• Biopharmaceutical Characterization: Critical for the structural analysis of monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), and other therapeutic proteins to assess purity, confirm structure, and identify post-translational modifications.
• Recombinant Protein Processing: Employed in downstream processing to cleave affinity tags (e.g., polyhistidine-tags, SUMO-tags) from expressed fusion proteins with high specificity.
• Peptide Synthesis & Modification: Facilitates the site-specific cleavage and modification of synthetic peptides during chemical synthesis processes.
• Cell Biology Studies: Applied in studies involving extracellular matrix proteins and cell surface receptors where lysine-specific cleavage is required.
• Diagnostic Kit Manufacturing: Used as a key enzymatic component in the production of certain in-vitro diagnostic (IVD) assays and kits.

Basic Information

Product Name	Recombinant Lysyl Endopeptidase
CAS No.	72561-05-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Lysyl Endopeptidase; Achromobacter Protease I; Lysobacter enzymogenes Endoproteinase Lys-C; Lysonase; Endoproteinase Lys-C; Protease from Achromobacter lyticus; Lys-C Protease; Peptidase K; EC 3.4.21.50
EINECS	Contact for details

Quality Control

Our recombinant Lysyl Endopeptidase is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and the absence of contaminating proteases. A comprehensive Certificate of Analysis (COA) detailing purity (by SDS-PAGE and HPLC), specific activity, and endotoxin levels is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. Keep the product in its original container under the recommended conditions to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (Activity)	Cleaves synthetic substrate specific for Lys-C protease
Purity (SDS-PAGE)	≥ 95%
Specific Activity	> 20 units/mg protein
Protease Contamination	No detectable trypsin, chymotrypsin, or other serine protease activity
Endotoxin Level	< 1.0 EU/μg (for research-grade)
Protein Concentration	As per COA (for solution form)
Storage Buffer	Typically 20 mM Tris-HCl, pH 8.0 (for solution form)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.