Description

Disuccinimidyl β-Hydromuconate is a specialized, heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a thiol-reactive maleimide group. This compound is critical for creating stable, covalent linkages between proteins, peptides, and other biomolecules, enabling advanced bioconjugation strategies. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceutical development, proteomics, and diagnostic assay production who require precise and efficient protein modification.

Application

• Bioconjugation and Protein Labeling: For covalently attaching fluorophores, enzymes, or other probes to antibodies, proteins, and peptides for diagnostic and research applications.
• Antibody-Drug Conjugate (ADC) Development: As a key linker molecule for connecting cytotoxic drugs to monoclonal antibodies in targeted cancer therapy research.
• Immobilization Chemistry: For the functionalization of surfaces, beads, or biosensors by linking biomolecules to solid supports via amine and thiol groups.
• Crosslinking for Structural Studies: Used in mass spectrometry and structural biology to study protein-protein interactions and complex formations.
• Polymer and Biomaterial Modification: To introduce functional groups or crosslink polymer chains in the development of advanced hydrogels and biocompatible materials.

Basic Information

Product Name	Disuccinimidyl β-Hydromuconate
CAS No.	72200-46-5
Molecular Formula	C14H12N2O8
Molecular Weight	336.26 g/mol
Synonyms	β-Hydromuconic Acid Disuccinimidyl Ester; DSBH; N,N'-Disuccinimidyl β-Hydromuconate; 3-Hexenedioic Acid, 1,6-Bis(2,5-dioxo-1-pyrrolidinyl) Ester; Bis(N-succinimidyl) β-Hydromuconate; 1,6-Bis(2,5-dioxopyrrolidin-1-yl)hex-3-enedioate; Heterobifunctional NHS-Maleimide Crosslinker
EINECS	Contact for details

Quality Control

Our Disuccinimidyl β-Hydromuconate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our clients in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.