Description

Pglu-Gly-Arg p-Nitroanilide Hydrochloride is a specialized chromogenic peptide substrate designed for the sensitive and specific detection of enzymatic activity. This compound is critical for biochemical research and diagnostic assay development, serving as a key reagent for quantifying protease and amidase activity. It is primarily utilized by pharmaceutical R&D teams, clinical diagnostics manufacturers, and academic research institutions focused on enzymology and high-throughput screening.

Application

• Protease Activity Assays: A primary substrate for the kinetic and colorimetric determination of trypsin, thrombin, and other serine proteases.
• Diagnostic Kit Manufacturing: Used as a core component in the production of clinical diagnostic kits for coagulation disorders and pancreatic function tests.
• Biochemical Research: Essential for studying enzyme kinetics, inhibition mechanisms, and substrate specificity in academic and industrial laboratories.
• Pharmaceutical Development: Employed in drug discovery pipelines for screening and characterizing potential therapeutic enzyme inhibitors.
• Quality Control in Biologics: Applied in the QC testing of biopharmaceuticals to monitor and validate the presence or absence of specific protease activities.

Basic Information

Product Name	Pglu-Gly-Arg p-Nitroanilide Hydrochloride
CAS No.	72194-52-6
Molecular Formula	C20H28N8O7 • HCl
Molecular Weight	529.95 g/mol
Synonyms	Pyrrolidonecarbonyl-Gly-Arg-pNA • HCl; L-Pyroglutamyl-Glycyl-L-arginine 4-nitroanilide hydrochloride; H-pGlu-Gly-Arg-pNA • HCl; Chromogenic Substrate S-2765 (related); Z-Gly-Arg-pNA • HCl (structural analog); N-α-Pyroglutamyl-glycyl-L-arginine-p-nitroanilide hydrochloride; Benzyloxycarbonyl-Gly-Arg-p-nitroanilide hydrochloride (common variant); Substrate for Factor Xa and Trypsin
EINECS	Contact for details

Quality Control

Our Pglu-Gly-Arg p-Nitroanilide Hydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is subjected to comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and functional activity testing. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Biological Activity	Conforms to functional assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.