Description

Ficoll Type 70 is a high-purity, synthetic, high-mass polymer of sucrose and epichlorohydrin, widely recognized for its utility in creating density gradients. This product is essential for the gentle and effective separation of cells, organelles, and other biological particles based on their buoyant density. It is a critical reagent for research and diagnostic laboratories in the life sciences, biotechnology, and pharmaceutical development sectors.

Application

• Density Gradient Centrifugation: Primary application for the isolation of lymphocytes, platelets, and other mammalian cells from whole blood or tissue homogenates.
• Cell Separation Media: Used in the preparation of Ficoll-Paque™-like solutions for one-step mononuclear cell isolation.
• Organelle Isolation: Effective in creating gradients for the purification of nuclei, mitochondria, and lysosomes from cell lysates.
• Virus and Vesicle Purification: Employed in the separation and concentration of exosomes, microvesicles, and certain viral particles.
• Molecular Biology: Serves as a stabilizing agent and viscosity enhancer in some nucleic acid handling and electrophoresis protocols.
• Diagnostic Reagent Formulation: A key component in in-vitro diagnostic (IVD) kits for hematological analysis and immunology testing.

Basic Information

Item	Details
Product Name	Ficoll Type 70
CAS No.	72146-89-5
Molecular Formula	(C6H10O5)n • (C3H5ClO)m
Molecular Weight	~70,000 (Average)
Synonyms	Ficoll 70; Poly(sucrose-co-epichlorohydrin); Sucrose-epichlorohydrin copolymer; Ficoll® 70; Polysucrose; Ficoll PM 70; Density gradient medium Ficoll 70
EINECS	Contact for details

Quality Control

Our Ficoll Type 70 is manufactured under a quality management system and undergoes rigorous analytical testing to ensure batch-to-batch consistency and performance in sensitive biological applications. Each lot is tested for critical parameters including molecular weight distribution, osmolality, pH, and endotoxin levels. A comprehensive Certificate of Analysis (COA) detailing purity, biological performance, and specification compliance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a dry environment to prevent moisture absorption, which can affect performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Assay (Dry Basis)	≥ 98.0 %
Average Molecular Weight	70,000 ± 10%
pH (10% solution)	5.0 - 7.0
Osmolality (10% solution)	Contact for details
Endotoxin	< 0.1 EU/mg
Loss on Drying	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.