Description

Suc-Ala-Ala-Phe-Amc CAS NO 71973-79-0 is a fluorogenic peptide substrate specifically designed for the sensitive and continuous assay of chymotrypsin and chymotrypsin-like proteases. This compound matters because it provides researchers and quality control labs with a reliable, high-throughput tool for enzymatic activity measurement, crucial for biochemical research and pharmaceutical development. It is primarily needed by professionals in academic research, pharmaceutical R&D, and diagnostic kit manufacturing for studying protease kinetics, inhibitor screening, and enzyme characterization.

Application

• Protease Activity Assays: Primary substrate for the fluorometric determination of chymotrypsin, cathepsin G, and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Biochemical Research: Essential tool for kinetic studies, enzyme mechanism elucidation, and specificity profiling in academic and industrial laboratories.
• Diagnostic Development: Employed in the development of clinical diagnostic assays that measure protease levels as disease biomarkers.
• Quality Control: Used in biopharmaceutical manufacturing to monitor the activity and consistency of enzyme-based products or processes.
• Cell Biology Studies: Applied in assays to detect and quantify protease activity within cellular and tissue extracts.

Basic Information

Product Name	Suc-Ala-Ala-Phe-Amc
CAS No.	71973-79-0
Molecular Formula	C27H33N5O8
Molecular Weight	555.58 g/mol
Synonyms	Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin; Suc-AAPF-AMC; Suc-AAPF-AMC; Suc-Ala-Ala-Phe-AMC; N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin; Chymotrypsin Substrate I (Fluorogenic); AAPF-AMC; N-Succinyl-L-alanyl-L-alanyl-L-phenylalanine 7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Suc-Ala-Ala-Phe-Amc is manufactured under strict quality protocols to ensure high purity and consistent performance for sensitive biochemical assays. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation. Certificates of Analysis (COA) detailing batch-specific results are available upon request to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in chymotrypsin assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.