Description

trans-4-(Maleimidomethyl)Cyclohexanecarboxylic Acid-Nhs is a high-purity, heterobifunctional crosslinking reagent featuring a maleimide group and an N-hydroxysuccinimide (NHS) ester. This compound is critically important for its ability to create stable, covalent bonds between thiol-containing molecules (like proteins or peptides) and primary amines under mild, aqueous conditions. It is an essential tool for researchers and manufacturers in the bioconjugation, diagnostics, and pharmaceutical development sectors who require precise and efficient linker chemistry.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: A key reagent for site-specific conjugation of cytotoxic drugs, fluorophores, or other payloads to monoclonal antibodies via cysteine residues.
• Protein and Peptide Labeling: Used to attach labels, tags (e.g., biotin, fluorescent dyes), or solid supports to proteins and peptides for detection, purification, or immobilization.
• Diagnostic Assay Development: Facilitates the stable coupling of enzymes, antibodies, or antigens in the manufacture of ELISA kits, lateral flow assays, and other immunoassays.
• Polymer and Biomaterial Functionalization: Enables the modification of polymers, hydrogels, and nanoparticles with bioactive ligands to create advanced drug delivery systems or tissue engineering scaffolds.
• Immobilization Chemistry: Used to covalently attach biomolecules to sensor chips (e.g., for Surface Plasmon Resonance), chromatography resins, or other surfaces while maintaining activity.
• Crosslinking for Structural Studies: Employed in structural biology to map protein-protein interactions or stabilize protein complexes for analysis by techniques like mass spectrometry.

Basic Information

Product Name	trans-4-(Maleimidomethyl)Cyclohexanecarboxylic Acid-Nhs
CAS No.	71875-81-5
Molecular Formula	C17H20N2O6
Molecular Weight	348.35 g/mol
Synonyms	trans-4-(Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester; 4-Maleimidomethyl-1-cyclohexanecarboxylic acid N-succinimidyl ester (trans); SMCC NHS ester; Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate; NHS-PEG4-Maleimide (core structure); Sulfo-SMCC precursor; Heterobifunctional crosslinker SMCC; Cyclohexane-NHS-Maleimide linker
EINECS	Contact for details

Quality Control

Our trans-4-(Maleimidomethyl)Cyclohexanecarboxylic Acid-Nhs is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize condensation and hydrolysis of the active NHS ester.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (NHS ester content)
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.