Description

Di-Sodium 4-Nitrophenyl Phosphate Hexahydrate is a high-purity chromogenic substrate specifically designed for the determination of alkaline and acid phosphatase enzyme activity. This compound is critical for accurate biochemical analysis and diagnostic testing, where precise measurement of enzymatic activity is required. It is an essential reagent for research laboratories, clinical diagnostics, and quality control in the pharmaceutical and biotechnology industries.

Application

• Clinical Diagnostics: Primary substrate in colorimetric assays for measuring alkaline phosphatase (ALP) and acid phosphatase (ACP) levels in serum, plasma, and other biological fluids, aiding in the diagnosis of liver, bone, and prostate disorders.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and characterization of phosphatase enzymes from various biological sources.
• Pharmaceutical QC: Employed in quality control testing of biopharmaceuticals and in cell-based assays during drug development to monitor cellular processes.
• Food & Environmental Analysis: Applied in test kits for the detection of phosphatase activity as an indicator of pasteurization efficacy in dairy products or for monitoring microbial contamination.
• In Vitro Diagnostics (IVD) Manufacturing: A key raw material in the production of reagent kits and automated analyzer systems for clinical chemistry.

Basic Information

Product Name	Di-Sodium 4-Nitrophenyl Phosphate Hexahydrate
CAS No.	71735-29-0
Molecular Formula	C6H4NNa2O6P · 6H2O
Molecular Weight	371.14 g/mol (Hexahydrate)
Synonyms	PNPP (p-Nitrophenyl Phosphate, Disodium Salt); 4-Nitrophenyl Phosphate Disodium Salt Hexahydrate; Phosphatase Substrate; pNPP; Disodium p-Nitrophenyl Phosphate; ALP Substrate; NSC 84200; PNPP, Disodium Salt
EINECS	Contact for details

Quality Control

Our Di-Sodium 4-Nitrophenyl Phosphate Hexahydrate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive enzymatic assays. Each lot is tested to meet stringent specifications for identity, purity, and suitability for use as a diagnostic substrate. Certificates of Analysis (COA) detailing purity (by HPLC), assay, absorbance characteristics, and enzymatic suitability are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Absorbance (405 nm, 1 cm)	≤ 0.05 (in assay buffer)
Water Content (KF)	26.0 - 31.0 % (Theoretical for Hexahydrate: 29.1%)
Solubility	Clear, colorless solution in water or assay buffer
Enzymatic Suitability	Suitable for phosphatase determination

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.