Description

n-Boc-L-Leucylglycyl-Arginine-p-Nitroanilide Hydrochloride is a specialized synthetic peptide substrate designed for high-precision biochemical research and diagnostic applications. Its core value lies in its role as a chromogenic substrate for the sensitive detection and kinetic study of specific proteolytic enzymes, such as trypsin and thrombin. This compound is essential for researchers and manufacturers in the fields of pharmaceutical development, clinical diagnostics, and proteomics, where reliable and consistent substrate performance is critical for assay accuracy and reproducibility.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of trypsin, thrombin, and related serine protease activity.
• Diagnostic Kit Manufacturing: Key raw material in the production of in-vitro diagnostic (IVD) kits for coagulation disorders and pancreatic function tests.
• Biochemical Research: Used in kinetic studies, enzyme inhibition screening, and mechanistic investigations of protease function.
• Pharmaceutical Quality Control: Employed in purity and potency testing of enzyme-based biopharmaceuticals during production.
• Proteomics and Cell Biology: A tool for studying protease signaling pathways and cellular processes involving specific proteolytic cleavage.

Basic Information

Product Name	n-Boc-L-Leucylglycyl-Arginine-p-Nitroanilide Hydrochloride
CAS No.	71730-95-5
Molecular Formula	C24H39N9O7 • HCl
Molecular Weight	602.09 g/mol
Synonyms	Boc-Leu-Gly-Arg-pNA • HCl; Boc-Leu-Gly-Arg-4-nitroanilide hydrochloride; L-Leucine, N-[(1,1-dimethylethoxy)carbonyl]glycyl-N5-(aminoiminomethyl)-N2-[4-(4-nitrophenyl)amino]-4-oxobutanoyl]-, hydrochloride (1:1); Trypsin Substrate Boc-Leu-Gly-Arg-pNA; Chromogenic Substrate for Trypsin; Thrombin Substrate; Protease Substrate 71730-95-5
EINECS	Contact for details

Quality Control

Our n-Boc-L-Leucylglycyl-Arginine-p-Nitroanilide Hydrochloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for critical research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation (IR, MS), and specific activity validation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For extended storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Enzymatic Activity	Validated for trypsin/thrombin assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.