Description

4-Nitrophenyl α-D-Glucuronide is a chromogenic substrate specifically designed for the detection and quantification of α-glucuronidase enzyme activity. This compound is critical for research and diagnostic applications where precise enzymatic measurement is required. It is primarily utilized by professionals in the life sciences, pharmaceutical development, and clinical diagnostics sectors.

Application

• Enzymatic Assay Substrate: Primary substrate for the spectrophotometric determination of α-glucuronidase activity in biochemical research.
• Diagnostic Reagent: Used in the development of diagnostic kits for detecting specific bacterial enzymes or genetic disorders related to glucuronide metabolism.
• Microbiology Research: Employed in studies of microbial flora, particularly for identifying bacterial species that express α-glucuronidase (e.g., certain strains of E. coli).
• Pharmaceutical Screening: Serves as a tool in high-throughput screening (HTS) platforms to identify potential inhibitors or activators of the α-glucuronidase enzyme for drug discovery.
• Biochemical Characterization: Essential for the kinetic analysis and characterization of α-glucuronidase enzymes purified from various biological sources.
• Quality Control in Enzyme Production: Used to standardize and assess the activity of commercially prepared α-glucuronidase batches.

Basic Information

Product Name	4-Nitrophenyl α-D-Glucuronide
CAS No.	71484-85-0
Molecular Formula	C12H13NO9
Molecular Weight	315.23 g/mol
Synonyms	p-Nitrophenyl α-D-Glucopyranosiduronic Acid; 4-Nitrophenyl α-D-Glucopyranosiduronic Acid; α-4-Nitrophenyl Glucuronide; PNPG; pNP-α-D-Glucuronide; 4-Nitrophenyl-α-D-glucuronide; α-4-NP-Glucuronide
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl α-D-Glucuronide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, methanol
Absorbance (Lambda max)	~400 nm (in alkaline solution)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.