Description

Bromoacetyl-Sepharose is a functionalized affinity chromatography medium designed for the covalent immobilization of ligands containing primary amino or thiol groups. This activated matrix is critical for researchers and manufacturers requiring robust, high-capacity separation and purification systems. It is primarily utilized in life sciences research, biopharmaceutical downstream processing, and diagnostic kit development for the isolation of antibodies, enzymes, and other biomolecules.

Application

• Affinity Chromatography Resin: For the purification of proteins, antibodies (IgG), enzymes, and recombinant proteins by coupling specific ligands.
• Immunoassay Development: As a solid support for immobilizing antigens or antibodies in diagnostic test kits and biosensors.
• Enzyme Immobilization: For creating stable, reusable biocatalysts for industrial enzymatic processes and bioreactors.
• Lectins and Sugar-Binding Protein Purification: By coupling appropriate glycosylated ligands for glycomics research.
• Nucleic Acid Binding Protein Isolation: For studying DNA-protein or RNA-protein interactions.
• Peptide Library Screening: Serving as a support matrix for combinatorial chemistry and drug discovery.
• Removal of Specific Contaminants: In process streams by coupling selective scavengers or inhibitors.

Basic Information

Product Name	Bromoacetyl-Sepharose
CAS No.	71372-91-3
Molecular Formula	N/A (Functionalized polymer matrix)
Molecular Weight	N/A (Cross-linked agarose bead derivative)
Synonyms	Bromoacetyl-Sepharose 4B; Sepharose 4B bromoacetyl; Bromoacetyl agarose; Activated CH-Sepharose 4B; N-Bromoacetyl-6-aminohexyl-Sepharose; Bromoacetylated Sepharose CL-4B; Affi-Gel bromoacetyl gel; Bromoacetyl-activated cross-linked agarose
EINECS	Contact for details

Quality Control

Our Bromoacetyl-Sepharose is manufactured under a quality management system. Each lot is tested to ensure consistent performance characteristics, including coupling capacity, bead size distribution, and flow properties. A Certificate of Analysis (COA) detailing specific batch data is provided with every shipment. We support compliance requirements for research and industrial applications.

Storage

Preserve in a tightly closed container, protected from light. Store as supplied in the original container at 2-8°C. Do not freeze. The suspension contains a preservative; handle with appropriate laboratory precautions. Keep away from strong bases and amines.

Specification

Item	Specification
Matrix	Cross-linked 4% Agarose (Sepharose 4B)
Bead Size Range	45-165 μm
Activation Level	10-30 μmol bromoacetyl groups / mL gel
Coupling Capacity	>15 mg protein / mL gel (for IgG)
Preservative	Contains 20% Ethanol
pH Stability (long term)	3-10 (coupled gel)
Chemical Stability	Stable in most aqueous buffers; avoid strong nucleophiles

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.