Description

3-(Glutathion-S-Yl)-n-Methyl-4-Aminoazobenzene is a specialized biochemical conjugate formed between glutathione and a methylated aminoazobenzene derivative. This compound is of significant interest in biochemical and toxicological research as a model adduct for studying the metabolic activation and detoxification pathways of aromatic amines and azo dyes. It serves as a critical reference standard and research tool for scientists investigating glutathione S-transferase activity, carcinogen metabolism, and DNA/protein adduct formation. Primary users include research institutions, pharmaceutical R&D departments, and contract research organizations (CROs) focused on molecular toxicology and biomarker discovery.

Application

• Biochemical Research Standard: Used as a high-purity reference compound for calibrating analytical instruments like HPLC-MS and LC-MS/MS in adductomics studies.
• Toxicology & Metabolic Studies: Serves as a model substrate or marker for investigating the metabolic fate, bioactivation, and detoxification mechanisms of aromatic amines and azo compounds.
• Enzyme Activity Assays: Employed in in vitro assays to study the kinetics and specificity of Glutathione S-Transferase (GST) enzymes.
• Biomarker Development: Acts as a synthetic analog for developing and validating analytical methods to detect specific glutathione-derived adducts in biological samples.
• Carcinogenesis Research: Utilized in studies exploring the link between chemical exposure, covalent adduct formation with cellular macromolecules, and the initiation of carcinogenesis.
• Pharmaceutical Safety Assessment: Applied in drug metabolism and pharmacokinetics (DMPK) studies to understand potential reactive metabolite formation and glutathione conjugation pathways of new chemical entities.

Basic Information

Product Name	3-(Glutathion-S-Yl)-n-Methyl-4-Aminoazobenzene
CAS No.	71259-45-5
Molecular Formula	C23H30N6O6S
Molecular Weight	518.59 g/mol
Synonyms	3-(GS-yl)-N-Methyl-4-aminoazobenzene; Glutathione-MAB Conjugate; S-[2-[[(4S)-4-Amino-5-[[(1R)-1-[[(carboxymethyl)amino]-1-oxo-3-sulfanylpropan-2-yl]amino]-5-oxopentyl]amino]-2-oxoethyl]-N-(4-methylphenyl)diazenecarbothioamide; N-Methyl-4-aminoazobenzene-glutathione conjugate; MAB-GSH Adduct; Azo Dye-Glutathione Metabolite; GST Substrate Analog
EINECS	Contact for details

Quality Control

Every batch of 3-(Glutathion-S-Yl)-n-Methyl-4-Aminoazobenzene is produced and analyzed under strict quality protocols to ensure identity, purity, and consistency suitable for advanced research applications. Our products undergo rigorous quality testing, including chromatographic purity analysis and structural confirmation, to ensure compliance with high-grade research standards. A comprehensive Certificate of Analysis (COA) detailing batch-specific results for identity, assay purity, and related substances is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. For long-term storage, consider storing desiccated at -20°C. Handle and store in accordance with good laboratory practices.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.