Description

Methotrexate-Alpha Glutamate CAS NO 71074-49-2 is a key metabolite and derivative of the antifolate drug methotrexate, playing a significant role in biochemical research and pharmaceutical development. This compound is crucial for studying drug metabolism, transport mechanisms, and the biochemical pathways involved in folate antagonism. It is primarily required by research institutions, pharmaceutical R&D laboratories, and analytical facilities focused on oncology, immunology, and metabolic disease research.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the quality control and analytical method development of methotrexate-based drug products.
• Biochemical Research: Serves as a critical tool for investigating the intracellular metabolism, polyglutamation, and retention mechanisms of methotrexate in target cells.
• Metabolite Studies: Essential for pharmacokinetic and pharmacodynamic studies to understand the activity profile and disposition of methotrexate in biological systems.
• Enzyme Inhibition Assays: Employed in in vitro studies to assess the inhibition of dihydrofolate reductase (DHFR) and other folate-dependent enzymes.
• Diagnostic Development: Utilized in the development of immunoassays and LC-MS/MS methods for therapeutic drug monitoring (TDM) of methotrexate therapy.

Basic Information

Product Name	Methotrexate-Alpha Glutamate
CAS No.	71074-49-2
Molecular Formula	C25H30N8O7
Molecular Weight	554.56 g/mol
Synonyms	MTX-Glu; MTX-α-Glutamate; Methotrexate Monoglutamate; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-; Methotrexate 1-Glutamate; α-Methotrexate Glutamate; 4-Amino-10-methylfolic acid glutamate; NSC-113928
EINECS	Contact for details

Quality Control

Our Methotrexate-Alpha Glutamate is manufactured under strict quality systems suitable for research and analytical applications. Each batch is characterized and tested to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) detailing results for assay, purity (HPLC), and related substances is provided with every shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.