Description

Boc-Ala-Ala-Phe-Pna CAS NO 70968-20-6 is a protected tetrapeptide derivative featuring a p-nitroanilide (Pna) chromogenic group. This compound serves as a critical substrate for the study and characterization of proteolytic enzymes, particularly serine and cysteine proteases. It is an essential research tool for pharmaceutical R&D, biochemistry, and enzymology laboratories focused on drug discovery, enzyme kinetics, and inhibitor screening.

Application

• Enzyme Substrate for Protease Assays: Primarily used as a chromogenic substrate to measure the activity of proteases like chymotrypsin, subtilisin, and related enzymes, where cleavage releases p-nitroaniline for spectrophotometric detection.
• Biochemical Research & Drug Discovery: Employed in high-throughput screening (HTS) platforms to identify and evaluate novel protease inhibitors as potential therapeutic agents.
• Enzyme Kinetics Studies: Facilitates the determination of kinetic parameters (Km, Vmax, kcat) for proteases, aiding in the understanding of enzyme mechanism and specificity.
• Quality Control in Biotechnology: Used to assess the purity and specific activity of enzyme preparations in biomanufacturing and diagnostic kit production.
• Academic & Institutional Research: A standard reagent in university and institutional labs for teaching and advanced research in enzymology and peptide chemistry.

Basic Information

Product Name	Boc-Ala-Ala-Phe-Pna
CAS No.	70968-20-6
Molecular Formula	C27H34N6O7
Molecular Weight	554.60 g/mol
Synonyms	Boc-Ala-Ala-Phe-pNA; tert-Butyloxycarbonyl-Alanyl-Alanyl-Phenylalanyl-p-nitroanilide; N-(tert-Butoxycarbonyl)-L-alanyl-L-alanyl-L-phenylalanyl 4-nitroanilide; Boc-AAF-pNA; Boc-Ala-Ala-Phe-4-nitroanilide; Substrate for Chymotrypsin; Protease Substrate Boc-AAF-pNA; Chymotrypsin Chromogenic Substrate
EINECS	Contact for details

Quality Control

Our Boc-Ala-Ala-Phe-Pna is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency suitable for sensitive biochemical applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 97.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.