Description

Suc-Ala-Ala-Pro-Leu-Pna is a chromogenic peptide substrate specifically designed for the detection and kinetic study of serine proteases. This compound matters as a critical research tool, enabling precise biochemical analysis of enzyme activity and inhibition. It is primarily needed by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic kit manufacturers for applications in drug discovery and enzymatic assay development.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of serine protease activity, including elastase and related enzymes.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: Essential for kinetic studies, determining parameters such as Km and Vmax, and investigating enzyme mechanism and specificity.
• Diagnostic Development: Employed in the development of clinical diagnostic tests that measure protease levels in biological samples.
• Quality Control in Biotechnology: Used to assess the purity and specific activity of enzyme preparations in biomanufacturing.

Basic Information

Product Name	Suc-Ala-Ala-Pro-Leu-Pna
CAS No.	70968-04-6
Molecular Formula	C30H43N7O9
Molecular Weight	645.71 g/mol
Synonyms	Suc-AAPL-pNA; N-Succinyl-Ala-Ala-Pro-Leu-p-nitroanilide; Suc-AAPL-pNA; Sucinyl-Alanyl-Alanyl-Prolyl-Leucyl-p-Nitroanilide; Elastase Substrate IV; N-Succinyl-Ala-Ala-Pro-Leu p-nitroanilide; AAPL-pNA; Suc-AAPL-pNA Substrate
EINECS	Contact for details

Quality Control

Our Suc-Ala-Ala-Pro-Leu-Pna is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by mass spectrometry, to ensure it meets the high standards required for sensitive biochemical research. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Conforms (via reference enzyme assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.