Description

o-Nitrophenyl β-D-Cellobioside Heptaacetate is a specialized chromogenic substrate used extensively in enzymatic assays. This compound is critical for researchers and manufacturers requiring precise measurement of cellulase and β-glucosidase activity. It is primarily utilized by the biotechnology, pharmaceutical, and biofuel industries for enzyme characterization, screening, and quality control processes.

Application

• Enzyme Activity Assays: As a chromogenic substrate for the specific detection and quantification of cellulase and β-glucosidase enzymes.
• Biochemical Research: Used in kinetic studies and mechanism-of-action research for carbohydrate-active enzymes.
• Industrial Enzyme Screening: Employed in high-throughput screening platforms to identify and optimize novel cellulolytic enzymes for biofuel production.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for enzyme deficiency studies.
• Quality Control in Biotechnology: Used to standardize and validate the activity of commercial enzyme preparations.

Basic Information

Product Name	o-Nitrophenyl β-D-Cellobioside Heptaacetate
CAS No.	70867-22-0
Molecular Formula	C34H39NO20
Molecular Weight	781.67 g/mol
Synonyms	4-Nitrophenyl β-D-cellobioside heptaacetate; ONP-β-D-cellobioside heptaacetate; 2-Nitrophenyl 4-O-β-D-glucopyranosyl-β-D-glucopyranoside heptaacetate; p-Nitrophenyl cellobioside heptaacetate (common misnomer); Cellobioside heptaacetate, o-nitrophenyl derivative; Chromogenic cellulase substrate; β-Glucosidase substrate, chromogenic; 1,2,3,6-Tetra-O-acetyl-4-O-(2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl)-β-D-glucopyranose 1-(2-nitrophenyl) acetate
EINECS	Contact for details

Quality Control

Our o-Nitrophenyl β-D-Cellobioside Heptaacetate is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee reproducible performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.