Description

6-Doxylstearoyl-Coenzyme A is a spin-labeled derivative of coenzyme A, specifically functionalized with a doxyl nitroxide radical at the 6-position of the stearoyl moiety. This compound serves as a critical molecular probe for investigating lipid metabolism, enzyme kinetics, and membrane dynamics using Electron Paramagnetic Resonance (EPR) spectroscopy. It is essential for researchers and developers in biochemistry, pharmaceutical R&D, and metabolic studies who require precise tools to track and analyze acyl-CoA interactions and related enzymatic pathways.

Application

• EPR Spectroscopy Studies: As a spin label for probing the structure, dynamics, and local environment of proteins and enzymes that bind acyl-CoA substrates.
• Enzyme Mechanism Analysis: Investigating the active sites and catalytic mechanisms of acyltransferases, synthetases, and other CoA-dependent enzymes.
• Membrane Biophysics Research: Studying lipid-protein interactions and the behavior of fatty acyl chains within model membranes or lipoproteins.
• Metabolic Pathway Tracing: Serving as a tagged substrate to monitor fatty acid activation and utilization in complex biochemical pathways.
• Pharmaceutical Development: Used in early-stage drug discovery to screen for inhibitors or modulators of key metabolic enzymes.
• Diagnostic Assay Development: Potential use in developing advanced diagnostic kits for metabolic disorders.

Basic Information

Product Name	6-Doxylstearoyl-Coenzyme A
CAS No.	70691-32-6
Molecular Formula	C39H68N7O18P3S
Molecular Weight	1048.0 g/mol (approx.)
Synonyms	6-Doxyl-stearoyl-CoA; 6-Doxylstearoyl Coenzyme A; Stearoyl-CoA, 6-doxyl-; Coenzyme A, 6-doxylstearoyl-; 6-DOXYL-stearoyl-coenzyme A; EPR-labeled stearoyl-CoA; Spin-labeled acyl-CoA; Nitroxide-labeled CoA derivative
EINECS	Contact for details

Quality Control

Our 6-Doxylstearoyl-Coenzyme A is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity and EPR spectroscopy to confirm radical integrity and labeling efficiency. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. The product is easily oxidized (store under inert atmosphere) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Aliquot contents upon first use to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC)	Conforms
Identification (EPR)	Characteristic nitroxide radical signal present
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (as CoA derivative)
Solubility	Soluble in water, buffer solutions, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.