Description

Udp-n-Acetylglucosamine--Dolichyl-Phosphate n-Acetylglucosaminephosphotransferase is a critical enzyme in the eukaryotic N-linked glycosylation pathway, specifically catalyzing the first committed step in the synthesis of the lipid-linked oligosaccharide (LLO) precursor. This compound matters for researchers and manufacturers focused on glycobiology, as it is essential for studying and modulating protein glycosylation, a key post-translational modification affecting protein folding, stability, and function. It is primarily needed by biotechnology and pharmaceutical companies engaged in advanced research, drug discovery, and the development of therapeutic proteins and biologics where precise glycosylation patterns are crucial for efficacy and safety.

Application

• Glycobiology Research: Fundamental studies on the N-linked glycosylation pathway, enzyme kinetics, and substrate specificity.
• Drug Discovery & Development: Target for identifying inhibitors or modulators of glycosylation in diseases like cancer, congenital disorders of glycosylation (CDGs), and viral infections.
• Biopharmaceutical Production: Used in in vitro systems to study and optimize the glycosylation of recombinant therapeutic proteins, antibodies, and vaccines.
• Enzymology Studies: Serves as a standard or reagent for assaying phosphotransferase activity and understanding dolichol phosphate cycle regulation.
• Diagnostic Development: Potential component in assays designed to diagnose metabolic disorders related to glycosylation defects.

Basic Information

Product Name	Udp-n-Acetylglucosamine--Dolichyl-Phosphate n-Acetylglucosaminephosphotransferase
CAS No.	70431-08-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	UDP-GlcNAc:Dolichyl-Phosphate N-Acetylglucosaminephosphotransferase; GPT (GlcNAc-1-phosphotransferase); ALG7; DPAGT1; Dolichyl-phosphate N-acetylglucosaminephosphotransferase; EC 2.7.8.15; N-Acetylglucosamine-1-phosphate transferase; GlcNAc-1-P transferase; UDP-N-acetyl-D-glucosamine:dolichyl-phosphate N-acetyl-D-glucosaminephosphotransferase
EINECS	Contact for details

Quality Control

Our enzyme products undergo rigorous quality testing to ensure high specific activity, purity, and batch-to-batch consistency essential for research reproducibility. Quality is verified through a panel of analytical methods including activity assays, SDS-PAGE, and protein concentration determination. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and storage buffer composition is provided with each shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. This material is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent moisture absorption, which can affect activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content	By Bradford or UV absorbance
Buffer Composition	Tris-HCl, Glycerol, DTT (typical)
Endotoxin Level	< 1.0 EU/µg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.