Description

Z-Pro-Arg-Amc Hcl is a fluorogenic peptide substrate designed for the sensitive detection and quantification of trypsin-like serine proteases. This compound is of critical importance in biochemical research and pharmaceutical development for studying enzyme kinetics, inhibitor screening, and high-throughput assay development. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries.

Application

• Protease Activity Assays: Fluorogenic substrate for the continuous, real-time monitoring of trypsin, thrombin, plasmin, and related serine proteases.
• High-Throughput Screening (HTS): Essential tool in drug discovery campaigns to identify and characterize novel protease inhibitors.
• Enzyme Kinetic Studies: Used to determine kinetic parameters (Km, Vmax, kcat) for precise enzyme characterization.
• Diagnostic Development: Component in the development of clinical assays for conditions involving dysregulated protease activity.
• Cell Biology Research: Investigation of protease-mediated signaling pathways and cellular processes.
• Quality Control Testing: Validation of recombinant protease activity and purity in biomanufacturing.

Basic Information

Product Name	Z-Pro-Arg-Amc Hcl
CAS No.	70375-23-4
Molecular Formula	C24H35ClN6O6
Molecular Weight	539.03 g/mol
Synonyms	Z-Pro-Arg-AMC hydrochloride; Benzyloxycarbonyl-Pro-Arg-7-amido-4-methylcoumarin hydrochloride; Z-PR-AMC HCl; Cbz-Pro-Arg-AMC HCl; Z-Pro-Arg-AMC * HCl; Boc-Pro-Arg-AMC HCl (common alternative); Fluorogenic substrate for trypsin; 7-Amido-4-methylcoumarin substrate Z-PR-AMC
EINECS	Contact for details

Quality Control

Our Z-Pro-Arg-Amc Hcl is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your regulatory and quality assurance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Tested for fluorogenic response with trypsin

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.