Description

L-Arg-7-Amino-4-Methylcoumarin is a specialized fluorogenic peptide substrate and a key biochemical reagent. This compound is critical for enzymatic activity assays, particularly for detecting and quantifying protease activity with high sensitivity and specificity. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in drug discovery and biochemical research. Its unique structure makes it an essential tool for studying enzyme kinetics and developing high-throughput screening assays.

Application

• Protease Activity Assays: As a fluorogenic substrate for trypsin, thrombin, and other serine proteases.
• Drug Discovery Screening: High-throughput screening (HTS) platforms to identify novel protease inhibitors.
• Biochemical Research: Studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial labs.
• Diagnostic Development: A key component in the formulation of diagnostic kits for clinical and research use.
• Cell Biology Studies: Used in assays to monitor proteolytic activity within cellular systems.
• Quality Control in Biologics: Testing for residual protease activity in biopharmaceutical manufacturing processes.

Basic Information

Product Name	L-Arg-7-Amino-4-Methylcoumarin
CAS No.	70274-89-4
Molecular Formula	C19H26N6O4
Molecular Weight	402.45 g/mol
Synonyms	L-Arginine 7-amido-4-methylcoumarin; Arg-AMC; L-Arginine-7-amido-4-methylcoumarin; Boc-L-Arg-AMC; AMC-L-Arg; 7-Amino-4-methylcoumarin-L-arginine; Boc-Arg-AMC; Substrate for Trypsin (AMC-based)
EINECS	Contact for details

Quality Control

Our L-Arg-7-Amino-4-Methylcoumarin is produced under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for biochemical reagents to guarantee reliable and reproducible performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.