Description

Gly-Arg-Amc (Hydrochloride) is a fluorogenic peptide substrate specifically designed for the detection and measurement of trypsin-like protease activity. This compound is critical for biochemical research and diagnostic assay development, offering high sensitivity and specificity. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for enzyme kinetics studies, high-throughput screening, and the development of clinical diagnostic kits.

Application

• Protease Activity Assays: A key substrate for quantifying the enzymatic activity of trypsin, thrombin, plasmin, and other serine proteases in research settings.
• High-Throughput Screening (HTS): Enables the rapid screening of large compound libraries for potential protease inhibitors in drug discovery programs.
• Diagnostic Kit Development: Used in the formulation of diagnostic assays for conditions related to dysregulated protease activity, such as coagulation disorders or inflammatory diseases.
• Biochemical Research: Essential for studying enzyme kinetics, substrate specificity, and the mechanistic pathways of proteolytic enzymes.
• Quality Control in Biologics: Employed in monitoring the activity and consistency of enzyme-based biopharmaceutical products during manufacturing.

Basic Information

Product Name	Gly-Arg-Amc (Hydrochloride)
CAS No.	70274-78-1
Molecular Formula	C16H28ClN7O4
Molecular Weight	417.89 g/mol
Synonyms	Glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride; Gly-Arg-AMC•HCl; Boc-Gly-Arg-AMC hydrochloride; L-Glycyl-L-arginine 4-methyl-7-coumarylamide hydrochloride; Trypsin Substrate Gly-Arg-AMC; GRA-AMC hydrochloride; Protease Fluorogenic Substrate V; Z-Gly-Arg-AMC hydrochloride (common variant)
EINECS	Contact for details

Quality Control

Every batch of Gly-Arg-Amc (Hydrochloride) is manufactured under strict quality management systems. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation via mass spectrometry, to ensure compliance with research-grade specifications. Certificates of Analysis (COA) detailing batch-specific results are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Functional Activity	Suitable for fluorogenic protease assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.