Description

Coelenterazine 400 A is a high-purity, synthetic luciferin derivative widely used as a critical substrate in bioluminescence assays. Its core value lies in enabling highly sensitive, non-radioactive detection for a variety of molecular and cellular analysis platforms. This product is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries who require reliable and consistent performance in their assay systems.

Application

• Bioluminescence Resonance Energy Transfer (BRET) Assays: A key substrate for studying protein-protein interactions, receptor-ligand binding, and intracellular signaling pathways in live cells.
• Reporter Gene Assays: Used with Renilla luciferase as a standard for dual-luciferase reporter systems to measure gene expression and promoter activity.
• High-Throughput Screening (HTS): Enables rapid, sensitive detection of drug candidates and bioactive compounds in automated pharmaceutical screening platforms.
• In Vivo Imaging: Serves as a substrate for deep-tissue bioluminescent imaging in small animal models for oncology and disease progression studies.
• ATP Detection & Cell Viability Assays: Utilized in kits for quantifying cellular ATP levels, a direct indicator of metabolic activity and cell health.
• Environmental Monitoring & Biosensors: Applied in the development of sensitive biosensors for detecting specific analytes or pathogens.

Basic Information

Product Name	Coelenterazine 400 A
CAS No.	70217-82-2
Molecular Formula	C26H21N3O2
Molecular Weight	407.47 g/mol
Synonyms	Coelenterazine 400a; Coelenterazine cp; DeepBlueC™; 2-Deoxycoelenterazine; 6-(4-Deoxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-7H-imidazo[3,2-a]pyrazin-3-one; Benzyl-coelenterazine; CLZN 400 A
EINECS	Contact for details

Quality Control

Our Coelenterazine 400 A is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere. This product is strictly light-sensitive (protect from all light exposure), easily oxidized (store under inert atmosphere), and hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescence Activity	Conforms to functional assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.