Description

p-Nitrophenyl Phosphate is a key chromogenic substrate widely used in enzymatic assays for the detection of alkaline phosphatase activity. Its high sensitivity and reliable colorimetric readout make it an indispensable reagent for research and diagnostic applications. This compound is essential for professionals in life sciences, clinical diagnostics, and pharmaceutical development who require precise biochemical detection methods.

Application

• Enzyme-Linked Immunosorbent Assay (ELISA): Primary substrate for detecting alkaline phosphatase-conjugated antibodies in diagnostic kits and research assays.
• Clinical Chemistry: Used in automated analyzers for measuring alkaline phosphatase levels in serum, a critical marker for liver and bone disorders.
• Molecular Biology Research: Essential for colorimetric detection in nucleic acid hybridization techniques, such as Southern and Northern blotting.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify enzyme inhibitors or activators for drug discovery.
• Microbiology: Used as a substrate in chromogenic media and tests for bacterial identification based on phosphatase activity.
• Biochemistry & Cell Biology: A standard tool for quantifying phosphatase activity in cell lysates, tissue extracts, and purified enzyme preparations.

Basic Information

Product Name	p-Nitrophenyl Phosphate
CAS No.	7515-15-3
Molecular Formula	C6H6NO6P
Molecular Weight	219.09 g/mol
Synonyms	PNPP; 4-Nitrophenyl phosphate; 4-Nitrophenyl dihydrogen phosphate; Phosphoric acid mono(4-nitrophenyl) ester; p-Nitrophenyl phosphoric acid; 4-NPP; pNPP; Nitrophenylphosphate
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl Phosphate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive diagnostic and research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay systems. We adhere to relevant quality management standards to support our global clientele in regulated industries.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture or light may lead to decomposition. Ensure containers are sealed tightly after each use to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Solubility	Clear, colorless solution in water or buffer
Water Content (KF)	≤ 2.0%
Absorbance Ratio (A400/A310)	≤ 0.05 (in 0.1M NaOH)
Enzymatic Activity	Suitable for use with alkaline phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.