Description

Methotrexate Disodium Salt is a water-soluble, folate antagonist antimetabolite widely used in pharmaceutical and research applications. Its primary value lies in its potent inhibitory action on dihydrofolate reductase, which is critical for DNA synthesis and cell replication. This compound is essential for manufacturers in the pharmaceutical industry, particularly for producing injectable formulations, and is a vital tool for researchers in oncology, immunology, and biochemistry.

Application

• Pharmaceutical Active Ingredient (API): Primary use in the manufacture of injectable chemotherapy drugs for treating various cancers, including leukemia, lymphoma, and osteosarcoma.
• Immunosuppressive Therapy: Key component in formulations for managing autoimmune diseases such as rheumatoid arthritis, psoriasis, and Crohn's disease.
• Chemical Reference Standard: Serves as a high-purity standard for analytical testing, quality control, and method validation in pharmaceutical laboratories.
• Biochemical Research: Used in cell culture and molecular biology studies to investigate folate metabolism, cell cycle inhibition, and mechanisms of drug resistance.
• Veterinary Medicine: Employed in oncological treatments for companion animals under veterinary supervision.

Basic Information

Product Name	Methotrexate Disodium Salt
CAS No.	7413-34-5
Molecular Formula	C20H20N8Na2O5
Molecular Weight	516.41 g/mol
Synonyms	Methotrexate Sodium; MTX Disodium; Amethopterin Disodium Salt; 4-Amino-10-methylfolic acid disodium salt; (2S)-2-[[4-[(2,4-Diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid disodium salt; NSC-740; Lantarel (brand name variant); Rheumatrex (brand name variant)
EINECS	231-063-9

Quality Control

Our Methotrexate Disodium Salt is manufactured and tested under strict quality management systems. We ensure compliance with major pharmacopoeial standards, including USP (United States Pharmacopeia) and EP (European Pharmacopoeia) monographs, where applicable. Every batch undergoes comprehensive analytical testing, including assay, impurity profiling, and residual solvent analysis. A detailed Certificate of Analysis (COA) is provided with each shipment to guarantee identity, purity, and safety for its intended use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	98.0% - 102.0% (on anhydrous basis)
Water Content (KF)	≤ 8.0 %
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 0.5%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines
Bacterial Endotoxins	< 1.0 EU/mg (for injectable grade)
Sterility	Passes test (for sterile grade)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.