Description

1-(4-Nitrophenyl)-N,N'-Diacetyl-3,6,3',4',6'-Penta-o-Acetylchitobioside is a specialized, high-purity synthetic derivative of chitobiose, a disaccharide unit of chitin. This compound serves as a critical chromogenic substrate and a key intermediate in advanced biochemical research and pharmaceutical development. It is primarily utilized by research institutions, diagnostic manufacturers, and pharmaceutical companies engaged in enzyme kinetics studies, particularly for lysozyme and other glycosidase assays.

Application

• Enzyme Substrate: Acts as a specific chromogenic substrate for the spectrophotometric assay and kinetic study of lysozyme (muramidase) activity.
• Biochemical Research: Used as a model compound in carbohydrate chemistry research to study glycosidase mechanisms, inhibition, and enzyme specificity.
• Diagnostic Reagent Manufacturing: Serves as a key raw material in the production of diagnostic kits for measuring lysozyme levels in biological fluids, which is a marker for certain medical conditions.
• Pharmaceutical Intermediate: Utilized in the synthesis of more complex glycoconjugates and potential therapeutic agents targeting carbohydrate-processing enzymes.
• Quality Control: Employed as a reference standard in pharmaceutical and biotechnology quality control laboratories to calibrate and validate lysozyme assay methods.

Basic Information

Product Name	1-(4-Nitrophenyl)-N,N'-Diacetyl-3,6,3',4',6'-Penta-o-Acetylchitobioside
CAS No.	7284-19-7
Molecular Formula	C34H43N3O21
Molecular Weight	829.71 g/mol
Synonyms	p-Nitrophenyl N,N'-diacetyl-β-chitobioside; PNP-(GlcNAc)2; 4-Nitrophenyl 2-acetamido-4-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-2-deoxy-β-D-glucopyranoside; p-Nitrophenyl N,N'-diacetyl-β-D-chitobioside; Chitobiose Octaacetate p-Nitrophenyl Glycoside; Lysozyme Substrate; 1-(4-Nitrophenyl)-N,N'-diacetylchitobioside peracetate
EINECS	Contact for details

Quality Control

Our 1-(4-Nitrophenyl)-N,N'-Diacetyl-3,6,3',4',6'-Penta-o-Acetylchitobioside is produced under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and NMR for structural confirmation, to ensure it meets the exacting standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and assay results.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details
Assay (HPLC)	95.0 - 105.0% (on anhydrous basis)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.