Description

p-Nitrophenyl β-D-N,N'-Diacetylchitobiose is a specialized chromogenic substrate used for the sensitive detection and quantification of chitinase and lysozyme enzyme activity. This compound is critical for research and development in biochemistry, enzymology, and microbiology, providing a reliable and measurable colorimetric readout. It is primarily utilized by researchers in academic institutions, pharmaceutical R&D departments, and biotechnology companies focused on enzyme kinetics, antifungal agent discovery, and studies of the chitin degradation pathway.

Application

• Enzyme Activity Assays: Primary substrate for the spectrophotometric determination of endo-chitinase and lysozyme activity.
• Biochemical Research: Used in kinetic studies to investigate enzyme mechanisms, inhibition, and substrate specificity.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify novel antifungal compounds that target chitin synthesis or degradation.
• Microbiological Studies: Facilitates research on plant-pathogen interactions and the role of chitinases in host defense mechanisms.
• Diagnostic Development: Serves as a key reagent in developing diagnostic kits for conditions or pathogens where chitinase levels are a biomarker.
• Quality Control: Used in biomanufacturing to monitor the activity of enzyme preparations used in industrial processes.

Basic Information

Product Name	p-Nitrophenyl β-D-N,N'-Diacetylchitobiose
CAS No.	7284-16-4
Molecular Formula	C28H41N3O17
Molecular Weight	667.64 g/mol
Synonyms	pNP-(GlcNAc)2; 4-Nitrophenyl N,N'-diacetyl-β-D-chitobioside; 4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-β-D-glucopyranoside; p-Nitrophenyl β-D-N,N'-diacetylchitobioside; Chitinase Substrate; PNP-GlcNAc2; 4-NP-β-D-(GlcNAc)2; Lysozyme Substrate
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-N,N'-Diacetylchitobiose is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzyme assays. We adhere to stringent quality management protocols to support the reliability required in pharmaceutical and academic research.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light may lead to decomposition and reduced performance. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO
Enzymatic Activity	Yields p-nitrophenol upon chitinase hydrolysis

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.