Description

L-[4-3H]Phenylalanine is a high-specific-activity tritium-labeled amino acid used as a radioactive tracer in life science research. It enables precise quantification and dynamic tracking of phenylalanine metabolism, protein synthesis, and transport mechanisms with exceptional sensitivity. This compound is essential for researchers in academic institutions, pharmaceutical R&D laboratories, and contract research organizations conducting radiolabeling studies, receptor binding assays, and pharmacokinetic investigations.

Application

• Radioligand binding assays to characterize neurotransmitter receptors and transporters
• In vitro and in vivo protein synthesis studies, including pulse-chase experiments in mammalian cell cultures
• Metabolic flux analysis of aromatic amino acid pathways in cancer, neurodegenerative, and metabolic disease models
• Development and validation of PET/SPECT radiotracer analogs (as a labeled precursor or reference standard)
• Quality control and calibration of liquid scintillation counting (LSC) systems and radio-HPLC detectors
• Radiopharmaceutical stability testing and degradation pathway analysis

Basic Information

Product Name	L-[4-3H]Phenylalanine
CAS No.	7279-74-5
Molecular Formula	C9H10NO2T (T = 3H)
Molecular Weight	166.21 g/mol (non-radioactive base); specific activity varies by batch (typically 30–120 Ci/mmol)
Synonyms	L-Phenylalanine-4-tritium; L-Phenylalanine-[4-3H]; (2S)-2-Amino-3-(4-tritio-phenyl)propanoic acid; L-4-Tritiophenylalanine; L-[4-3H]Phe; L-Phenylalanine-4-T; [4-3H]L-Phenylalanine; (S)-2-Amino-3-(p-tritio-phenyl)propionic acid
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is verified for radiochemical purity (>98% by radio-HPLC), specific activity (certified range provided), enantiomeric purity (>99% L-isomer by chiral HPLC), and absence of microbial contamination. Certificates of Analysis (COA) are available upon request and include full traceability, decay-corrected assay data, and supporting chromatograms. Manufacturing adheres to ISO 9001-certified quality management practices and follows principles aligned with current Good Manufacturing Practice (cGMP) for research-use-only radiolabeled compounds.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent radiolytic degradation and oxidation. Avoid repeated freeze-thaw cycles. Maintain low humidity environment to minimize potential hydrolysis.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear colorless solution in aqueous ethanol
Radiochemical Purity (by radio-HPLC)	≥98%
Enantiomeric Purity (by chiral HPLC)	≥99% L-isomer
Specific Activity	30–120 Ci/mmol (batch-specific, decay-corrected to date of release)
pH (1 mg/mL in water)	5.0–7.0
Identification (IR)	Conforms to reference spectrum
Assay (radio-HPLC)	95–105% of labeled claim

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.