Description

7-Hydroxydichloromethotrexate is a key chemical intermediate and reference standard in advanced pharmaceutical research and development. This compound is of significant interest for its role in the synthesis and metabolic study of methotrexate analogs, which are critical in therapeutic areas such as oncology and autoimmune diseases. It is primarily utilized by research institutions, pharmaceutical manufacturers, and contract development organizations engaged in the creation of novel chemotherapeutic agents and targeted therapies.

Application

• Pharmaceutical Intermediate: A crucial building block in the synthesis of novel methotrexate derivatives and folate antagonists for cancer treatment.
• Metabolite Reference Standard: Used as an analytical standard in pharmacokinetic and metabolic pathway studies for methotrexate-based drugs.
• Biochemical Research: Employed in enzymatic studies, particularly those involving dihydrofolate reductase (DHFR) inhibition assays.
• Active Pharmaceutical Ingredient (API) Development: Serves as a precursor in the development and scale-up of potential new active substances.
• Quality Control & Analytical Testing: Provides a certified reference material for HPLC, LC-MS, and NMR methods to ensure batch consistency and purity in manufacturing.

Basic Information

Product Name	7-Hydroxydichloromethotrexate
CAS No.	751-75-7
Molecular Formula	C₂₀H₂₂Cl₂N₈O₅
Molecular Weight	525.34 g/mol
Synonyms	7-Hydroxy-4-amino-10-methylpteroyl-γ-glutamic acid dichloro derivative; 7-OH-Dichloromethotrexate; NSC 139490; 4-Amino-10-methyl-7-hydroxy-pteroylglutamic acid dichloro analog; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-, 7-hydroxy dichloro derivative
EINECS	Contact for details

Quality Control

Our 7-Hydroxydichloromethotrexate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identity confirmation by IR and NMR, purity assessment by HPLC, and control of specified impurities. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with your research or development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container should be kept in a desiccated environment to prevent moisture absorption. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%
Single Unknown Impurity (HPLC)	≤ 0.5%
Total Impurities (HPLC)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.