Description

4-Nitrophenyl Phosphate, Bis(Cyclohexylammonium) Salt Hydrate is a high-purity biochemical reagent widely used as a chromogenic substrate for alkaline phosphatase (ALP) and other phosphatase enzymes. Its core value lies in its ability to produce a measurable colorimetric signal upon enzymatic hydrolysis, enabling sensitive detection and quantification in diagnostic and research assays. This compound is essential for manufacturers and research laboratories in the in-vitro diagnostics (IVD), life science research, and pharmaceutical development sectors, where reliable enzyme activity measurement is critical.

Application

• Clinical Diagnostics: Primary substrate in ELISA and other immunoassays for detecting alkaline phosphatase-labeled antibodies.
• Molecular Biology Research: Used in enzyme kinetics studies and high-throughput screening (HTS) for phosphatase activity.
• IVD Kit Manufacturing: A key raw material in the production of diagnostic test kits for various diseases, including bone and liver disorders.
• Biochemical Assay Development: Employed in developing colorimetric assays for drug discovery and biochemical pathway analysis.
• Quality Control (QC) Testing: Used in QC laboratories to validate the activity of alkaline phosphatase enzymes used in bioprocessing.
• Food Safety Testing: Potential application in assays designed to detect bacterial contamination via associated phosphatase activity.

Basic Information

Product Name	4-Nitrophenyl Phosphate, Bis(Cyclohexylammonium) Salt Hydrate
CAS No.	698999-89-2
Molecular Formula	C18H40N3O7P • xH2O
Molecular Weight	441.51 g/mol (anhydrous basis)
Synonyms	p-Nitrophenyl Phosphate, Disodium Salt (alternative form); PNPP; 4-NPP; Phosphoric Acid 4-Nitrophenyl Ester Bis(Cyclohexylammonium) Salt; Alkaline Phosphatase Substrate; PNPP, Disodium Salt, Hexahydrate (common commercial grade); 4-Nitrophenyl Phosphate Dicyclohexylammonium Salt Hydrate; pNPP
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Phosphate, Bis(Cyclohexylammonium) Salt Hydrate is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for absorbance characteristics, and enzymatic activity validation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0% (anhydrous basis)
Water Content (KF)	≤ 5.0% w/w
Absorbance (1% Solution)	Meets internal standard for low background
Enzymatic Suitability	Passes performance test with Alkaline Phosphatase
Solubility	Freely soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.