Description

4-Fluoro-2-Nitrophenyl β-D-Galactopyranoside is a specialized chromogenic and fluorogenic substrate for the enzyme β-galactosidase. This compound matters for its critical role in molecular biology and diagnostic assays, where it is used to detect and quantify enzymatic activity with high sensitivity. It is primarily needed by research institutions, pharmaceutical developers, and diagnostic kit manufacturers working in areas such as gene expression analysis, reporter gene assays, and microbiology.

Application

• Reporter Gene Assays: A key substrate for detecting β-galactosidase activity in lacZ gene expression studies, commonly used in molecular biology and genetics research.
• Diagnostic Development: Utilized in the formulation of diagnostic kits for bacterial detection (e.g., E. coli) and other enzymatic activity-based tests.
• Enzyme Kinetics & Inhibition Studies: Serves as a specific substrate for studying the kinetics of β-galactosidase and screening for potential enzyme inhibitors in drug discovery.
• Cell & Tissue Staining: Used in histochemistry and cytochemistry for visualizing β-galactosidase activity in cells and tissues, including applications in senescence assays.
• High-Throughput Screening (HTS): Employed in automated screening platforms within pharmaceutical and biotechnology companies to identify modulators of gene expression or enzymatic function.
• Microbiology Research: Acts as a differential substrate in microbial culture media for the identification of bacterial species expressing β-galactosidase.

Basic Information

Product Name	4-Fluoro-2-Nitrophenyl β-D-Galactopyranoside
CAS No.	694439-20-8
Molecular Formula	C12H14FNO8
Molecular Weight	319.24 g/mol
Synonyms	4-Fluoro-2-nitrophenyl β-D-galactopyranoside; 2-Nitro-4-fluorophenyl β-D-galactopyranoside; 4-F-2-NPG; F-2-Nitrophenyl-Gal; β-Galactosidase substrate 4-Fluoro-2-Nitrophenyl; p-Fluoro-o-nitrophenyl-β-D-galactoside; ONPF-β-Gal; Galactopyranoside, 4-fluoro-2-nitrophenyl, β-D-
EINECS	Contact for details

Quality Control

Our 4-Fluoro-2-Nitrophenyl β-D-Galactopyranoside is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for fine chemicals and can provide material tailored to specific research-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.