Description

n-Succinimidyloxycarbonylpropyl Methanethiosulfonate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a thiol-reactive methanethiosulfonate (MTS) group. This compound is engineered for the controlled and site-specific conjugation of biomolecules, enabling the creation of stable, well-defined protein-protein or protein-ligand complexes. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and protein labeling, where precise linkage chemistry is paramount.

Application

• Antibody-Drug Conjugate (ADC) Linker Chemistry: For the site-specific attachment of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein-Protein Crosslinking: Enables the study of protein interactions and the construction of multi-protein assemblies.
• Surface Immobilization: Functionalizing gold surfaces, biosensors, or chips with proteins or peptides through thiol-gold chemistry.
• Fluorescent or Biotin Labeling of Proteins: Conjugating reporter molecules (e.g., fluorophores, biotin) to specific cysteine sites on target proteins.
• PEGylation: Site-directed attachment of polyethylene glycol (PEG) chains to therapeutic proteins to enhance pharmacokinetics.
• Stable Complex Formation for Structural Biology: Creating covalently linked complexes for analysis by techniques like X-ray crystallography or cryo-EM.
• Diagnostic Reagent Development: Used in the manufacture of enzyme conjugates and other labeled reagents for immunoassays.

Basic Information

Product Name	n-Succinimidyloxycarbonylpropyl Methanethiosulfonate
CAS No.	690632-55-4
Molecular Formula	C9H13NO6S2
Molecular Weight	295.33 g/mol
Synonyms	SPDP Analog MTS; 3-((2,5-Dioxopyrrolidin-1-yl)oxy)-3-oxopropyl methanesulfonothioate; Methanesulfonothioic acid S-[3-[(2,5-dioxo-1-pyrrolidinyl)oxy]-3-oxopropyl] ester; NHS-PEG3-MTS; N-Succinimidyl 3-(methylsulfonylthio)propionate; MTS-SPDP; Heterobifunctional Crosslinker (NHS/MTS); Succinimidyl 3-(methanethiosulfonato)propionate
EINECS	Contact for details

Quality Control

Our n-Succinimidyloxycarbonylpropyl Methanethiosulfonate is manufactured under strict quality protocols to ensure batch-to-batch consistency and high reactivity. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and functional group assay. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes. We support development and cGMP manufacturing needs with appropriate documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white crystalline solid or powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.