Description

Rhod-2 (Potassium Salt) is a high-purity, cell-permeant fluorescent calcium indicator widely used in life science research. Its core value lies in its high selectivity for calcium ions (Ca²⁺) and its long-wavelength excitation and emission, which minimizes cellular autofluorescence and photodamage. This product is essential for researchers in neurobiology, cardiology, and cell physiology who require precise measurement of intracellular calcium dynamics. It is supplied as a stable, ready-to-use salt for consistent experimental results.

Application

• Intracellular Calcium Imaging: Primary application for real-time visualization and quantification of cytosolic Ca²⁺ transients in live cells using fluorescence microscopy.
• Neuroscience Research: Monitoring neuronal activity, synaptic transmission, and calcium signaling in neurons and glial cells.
• Cardiovascular Studies: Investigating calcium handling, excitation-contraction coupling, and arrhythmogenesis in cardiomyocytes.
• Cell Signaling Pathways: Studying the role of calcium as a second messenger in response to hormones, growth factors, and other stimuli.
• High-Throughput Screening (HTS): Utilized in plate reader-based assays for drug discovery targeting calcium channels or related pathways.
• Confocal and Multiphoton Microscopy: Ideal for deep-tissue imaging due to its favorable photophysical properties and reduced phototoxicity.

Basic Information

Product Name	Rhod-2 (Potassium Salt)
CAS No.	663625-18-1
Molecular Formula	C₄₆H₄₈K₂N₄O₂₃
Molecular Weight	1111.19 g/mol
Synonyms	Rhod-2, K⁺ salt; Rhod-2 potassium salt; X-Rhod-1; 5-(and-6)-Carboxy-X-rhodamine, bis(potassium salt); Calcium indicator Rhod-2; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-phenyl-3H-xanthen-9-yl)-2-oxoethoxy]-2-[2-amino-5-[carboxy(2-carboxylatophenyl)methyl]amino]-2-oxoethoxy]ethane-1,2-diyl bis[2-[bis(carboxymethyl)amino]acetate] dipotassium salt
EINECS	Contact for details

Quality Control

Our Rhod-2 (Potassium Salt) is manufactured under strict quality control protocols to ensure the high purity and batch-to-batch consistency required for sensitive research applications. Each lot is analyzed for identity, purity, and performance in standard calcium buffering systems. A comprehensive Certificate of Analysis (COA) detailing HPLC purity, absorbance/fluorescence spectra, and functional performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Absorbance (λmax)	549 - 553 nm
Emission (λmax)	576 - 580 nm
Calcium Binding Affinity (Kd)	~570 nM (in typical intracellular conditions)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.