Description

2,5-Dioxopyrrolidin-1-Yl 4-Methyl-4-((5-Nitropyridin-2-Yl)Disulfanyl)Pentanoate is a specialized heterobifunctional crosslinking agent featuring a disulfide bond and an activated ester. This compound is engineered for the controlled conjugation of biomolecules, offering a cleavable linkage under reducing conditions. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for advanced drug development, including antibody-drug conjugates (ADCs) and targeted therapeutic platforms.

Application

• Bioconjugation Reagent: A key intermediate for linking peptides, proteins, or antibodies to cytotoxic payloads, fluorophores, or other functional molecules.
• Antibody-Drug Conjugate (ADC) Development: Enables the construction of cleavable linkers in ADC platforms, facilitating targeted drug delivery and release.
• Proteomics Research: Used in chemical proteomics for labeling and studying protein interactions, dynamics, and localization.
• Diagnostic Reagent Synthesis: Employed in the preparation of enzyme-linked or fluorescent diagnostic probes and assays.
• Polymer and Material Science: Serves as a functional monomer or crosslinker for creating smart, stimuli-responsive biomaterials and hydrogels.
• Chemical Biology Tool: Facilitates site-specific modification of biomolecules to study structure-function relationships and cellular pathways.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 4-Methyl-4-((5-Nitropyridin-2-Yl)Disulfanyl)Pentanoate
CAS No.	663598-98-9
Molecular Formula	C15H15N3O6S2
Molecular Weight	397.42 g/mol
Synonyms	NHS-PEG4-SS-Pyridine Nitro; 4-Methyl-4-((5-nitropyridin-2-yl)disulfanyl)pentanoic acid 2,5-dioxopyrrolidin-1-yl ester; N-Succinimidyl 4-((5-nitropyridin-2-yl)disulfanyl)-4-methylpentanoate; SS-Pyridine Nitro NHS ester; Heterobifunctional Crosslinker (NHS ester/SS-Pyridine Nitro); 2,5-Pyrrolidinedione, 1-[[4-methyl-4-[(5-nitro-2-pyridinyl)dithio]-1-oxopentyl]oxy]-; 663598-98-9 (CAS)
EINECS	Contact for details

Quality Control

Our 2,5-Dioxopyrrolidin-1-Yl 4-Methyl-4-((5-Nitropyridin-2-Yl)Disulfanyl)Pentanoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.