Description

Butanoic Acid, 4-[(5-Nitro-2-Pyridinyl)Dithio]-, 2,5-Dioxo-3-Sulfo-1-Pyrrolidinyl Ester is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation and protein modification workflows. Its core value lies in its unique combination of a cleavable disulfide linker and an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester, enabling the controlled formation of reversible bonds under mild reducing conditions. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, and diagnostic assay creation, where precise and reversible protein labeling is required.

Application

• Bioconjugation Reagent: For the controlled, reversible crosslinking of proteins, antibodies, or peptides to other biomolecules, surfaces, or solid supports.
• Affinity Chromatography Ligand Synthesis: Used to immobilize ligands onto chromatographic resins via a cleavable disulfide bond for gentle elution of captured biomolecules.
• Drug-Linker Conjugate Development: Serves as a key intermediate in the synthesis of antibody-drug conjugates (ADCs) or other targeted therapeutics featuring a reducible linker system.
• Surface Plasmon Resonance (SPR) & Biosensor Fabrication: Enables the reversible attachment of capture molecules to sensor chips for regeneration and reuse.
• Protein Structure & Interaction Studies: Facilitates the analysis of protein complexes by introducing reversible crosslinks that can be dissected for analysis.
• Diagnostic Kit Component: Employed in the development of immunoassays where signal amplification or controlled reagent binding is necessary.

Basic Information

Product Name	Butanoic Acid, 4-[(5-Nitro-2-Pyridinyl)Dithio]-, 2,5-Dioxo-3-Sulfo-1-Pyrrolidinyl Ester
CAS No.	663598-89-8
Molecular Formula	C14H14N4O9S3
Molecular Weight	502.47 g/mol
Synonyms	4-[(5-Nitro-2-pyridinyl)dithio]butanoic acid 2,5-dioxo-3-sulfo-1-pyrrolidinyl ester; 3-Sulfo-NHS-SS-Pyridine-5-Nitro; Sulfo-NHS-SS-Pyridine (5-Nitro); 5-Nitro-2-pyridyl SS-sulfo-NHS ester; Heterobifunctional crosslinker, sulfo-NHS-SS-Pyridine; N-Sulfosuccinimidyl 3-[(2-nitro-5-pyridinyldithio]propionate; SPDP sulfonate analog; Water-soluble NHS-SS-Pyridine crosslinker
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical reagent adheres to stringent quality protocols. Every batch is characterized and tested to ensure consistent performance and lot-to-lot reproducibility for critical research and development applications. Key parameters including purity (by HPLC), functional group integrity, and solubility are verified. A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and the active ester is susceptible to hydrolysis. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO, buffer solutions
Functional Activity	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.