Description

Ac-Arg-Gly-Lys(Ac)-Amc is a specialized, fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like trypsin and related serine proteases. It is essential for applications in pharmaceutical R&D, biochemical research, and diagnostic kit development where accurate kinetic data is paramount.

Application

• Protease Activity Assays: Primary substrate for fluorometrically monitoring the activity of trypsin, thrombin, plasmin, and other serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel protease inhibitors or activators.
• Biochemical Research: A key tool for studying enzyme kinetics, specificity, and catalytic mechanisms in academic and industrial laboratories.
• Diagnostic Development: Employed in the development of clinical diagnostic tests that measure protease levels as disease biomarkers.
• Quality Control in Biopharma: Used to assess the purity and specific activity of enzyme preparations during manufacturing processes.
• Cell Biology Studies: Applied in assays to detect and quantify protease activity within cellular and tissue extracts.

Basic Information

Product Name	Ac-Arg-Gly-Lys(Ac)-Amc
CAS No.	660846-97-9
Molecular Formula	C30H46N10O8
Molecular Weight	674.76 g/mol
Synonyms	Ac-RGK(Ac)-AMC; N-Acetyl-Arg-Gly-Lys(Ac)-7-Amino-4-methylcoumarin; Acetyl-Arg-Gly-Lys(Acetyl)-AMC; Fluorogenic Substrate for Trypsin; Boc-Gly-Pro-Arg-AMC related substrate; Trypsin Substrate IV (related); Serine Protease Fluorogenic Peptide Substrate; (Acetyl-L-arginyl-glycyl-Nε-acetyl-L-lysyl)-7-amino-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Ac-Arg-Gly-Lys(Ac)-Amc is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via mass spectrometry. Certificates of Analysis (COA) detailing batch-specific results are available upon request. We support compliance standards relevant to research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.