Description

5'-O-(Dimethoxytrityl)-5-(1-Propynyl)-2'-Deoxyuridine is a high-purity, protected nucleoside derivative essential for advanced oligonucleotide synthesis. This compound serves as a critical building block for the site-specific incorporation of modified bases, enabling the creation of probes and therapeutic oligonucleotides with enhanced stability and binding properties. It is primarily required by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions focused on antisense technology, siRNA, and molecular diagnostics.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of modified DNA and RNA strands.
• Antisense & siRNA Development: Used to introduce the 5-propynyl-uracil modification, which can enhance duplex stability and nuclease resistance in therapeutic oligonucleotides.
• Molecular Probe Design: Enables the creation of labeled or functionalized probes for diagnostic assays, FISH, and microarray applications.
• Structural Biology Studies: Employed in research to investigate nucleic acid structure, function, and protein-nucleic acid interactions using modified bases.
• Apitamer Development: Facilitates the SELEX process by providing a modified nucleotide library to generate aptamers with improved binding affinity and specificity.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-5-(1-Propynyl)-2'-Deoxyuridine
CAS No.	644962-74-3
Molecular Formula	C30H30N2O7
Molecular Weight	530.57 g/mol
Synonyms	5'-O-DMT-5-(1-Propynyl)-2'-deoxyuridine; 5-Propynyl-dU DMT; DMT-5-Propynyl-dU; 5-(1-Propynyl)-2'-deoxyuridine, 5'-O-(4,4'-dimethoxytrityl) derivative; 5-Propynyl-2'-deoxyuridine DMT Protected; N/A
EINECS	Contact for details

Quality Control

Our production of 5'-O-(Dimethoxytrityl)-5-(1-Propynyl)-2'-Deoxyuridine adheres to stringent quality protocols suitable for research and pharmaceutical intermediate applications. Every batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.