Description

4-Methylumbelliferyl 4-Deoxy-β-D-Chitobioside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of chitinase and related enzymatic activity. This compound is critical for researchers and industrial scientists requiring precise biochemical analysis and high-throughput screening. It is primarily utilized in life science research, pharmaceutical development, and biotechnology applications focused on enzyme kinetics, inhibitor studies, and diagnostic assay development.

Application

• Chitinase Activity Assays: A key fluorogenic substrate for the continuous, sensitive measurement of chitinase and endo-β-N-acetylglucosaminidase activity in various samples.
• High-Throughput Screening (HTS): Enables the rapid screening of compound libraries for chitinase inhibitors or activators in drug discovery programs targeting fungal infections or insect control.
• Biochemical Research: Used in fundamental research to study enzyme kinetics, substrate specificity, and the mechanistic pathways of glycosyl hydrolases.
• Diagnostic Development: Serves as a core component in developing diagnostic kits for detecting pathogenic fungi or for environmental monitoring of chitinolytic organisms.
• Agricultural Biotechnology: Applied in research aimed at developing novel biocontrol agents or understanding plant-pathogen interactions involving chitin degradation.
• Quality Control in Enzyme Production: Used to standardize and quantify the activity of commercial chitinase preparations.

Basic Information

Product Name	4-Methylumbelliferyl 4-Deoxy-β-D-Chitobioside
CAS No.	618446-98-3
Molecular Formula	C24H32N2O13
Molecular Weight	556.52 g/mol
Synonyms	4-MU-4-Deoxy-chitobiose; 4-Methylumbelliferyl 4-deoxy-chitobioside; 4-MU-(GlcNAc)2 derivative; 4-Methylumbelliferyl β-D-N,N'-diacetylchitobioside analog; Fluorogenic chitobioside substrate; 4-MU-β-D-Chitobioside, 4-deoxy-; 7-Hydroxy-4-methylcoumarin 4-deoxy-β-D-chitobioside
EINECS	Contact for details

Quality Control

Every batch of 4-Methylumbelliferyl 4-Deoxy-β-D-Chitobioside is produced and tested under strict quality management protocols to ensure high purity and consistent performance. Our quality assurance includes comprehensive analytical characterization using HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and suitability for sensitive enzymatic assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Fluorogenic Activity	Functional in standard chitinase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.