Description

5'-Amino-Modifier-5 Cep is a specialized phosphoramidite monomer used for the solid-phase synthesis of oligonucleotides. This compound is essential for introducing a primary amine group at the 5'-terminus of DNA or RNA strands, enabling subsequent conjugation to dyes, quenchers, biotin, or other functional molecules. It is a critical reagent for researchers and manufacturers in the fields of molecular diagnostics, next-generation sequencing, and therapeutic oligonucleotide development who require precise and reliable site-specific labeling.

Application

• Oligonucleotide Labeling: Primary application for introducing a reactive amine handle at the 5' end of synthetic DNA/RNA for conjugation to fluorophores (e.g., Cy3, Cy5, FAM), biotin, or other probes.
• Diagnostic Probe Synthesis: Manufacturing of labeled probes for techniques like FISH (Fluorescence In Situ Hybridization), microarrays, and real-time PCR (qPCR).
• Therapeutic Oligonucleotide Modification: Site-specific functionalization of antisense oligonucleotides (ASOs), siRNA, and aptamers for targeted delivery or enhanced stability.
• Next-Generation Sequencing (NGS): Preparation of amine-modified adapters or primers for immobilization on flow cell surfaces or attachment of sequencing barcodes.
• Biosensor Development: Fabrication of oligonucleotide-based sensors by immobilizing amine-modified capture strands onto activated surfaces (e.g., glass, gold, carboxylated beads).
• Affinity Purification Systems: Creation of bait strands for pull-down assays by conjugating the amine-modified oligonucleotide to NHS-activated sepharose or other solid supports.

Basic Information

Product Name	5'-Amino-Modifier-5 Cep
CAS No.	612548-86-4
Molecular Formula	C41H49N4O7P
Molecular Weight	740.83 g/mol
Synonyms	5'-Amino-Modifier C6 Phosphoramidite; 5'-Amino-Modifier 5; 6-[(4-Monomethoxytrityl)amino]hexyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5'-Amino Modifier C6; 5'-Amino Hexyl Linker Phosphoramidite; Amino C6 Phosphoramidite; 5'-NH2 C6 Phosphoramidite; 6-(4-Monomethoxytritylamino)hexyl-1-[(2-cyanoethyl)-(N,N-diisopropylphosphoramidite)]
EINECS	Contact for details

Quality Control

Our 5'-Amino-Modifier-5 Cep is manufactured under strict quality-controlled conditions to ensure high purity and batch-to-batch consistency, suitable for demanding oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to industry best practices and can support requirements for GMP-grade manufacturing upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below under an inert atmosphere (argon or nitrogen) to maintain stability. This product is hygroscopic (moisture-sensitive) and easily oxidized; allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC/MS, 31P NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.