Description

Z-Phe-Val-Arg-Pna Hcl is a synthetic peptide substrate designed for high-specificity enzymatic activity studies. This compound is critical for biochemical research and pharmaceutical development, particularly in the study of serine proteases and related enzymatic pathways. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in drug discovery and quality control processes.

Application

• Protease Research & Assay Development: A key chromogenic substrate for studying the kinetics and inhibition of serine proteases like thrombin, trypsin, and related enzymes.
• Diagnostic Reagent Manufacturing: Used in the formulation of in-vitro diagnostic (IVD) kits for coagulation testing and other clinical assays.
• Pharmaceutical R&D: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors as potential drug candidates.
• Biochemical Standardization: Serves as a reference standard for calibrating enzymatic activity measurements in quality control laboratories.
• Academic Research: A fundamental tool in university and institutional labs for investigating proteolytic mechanisms and signal transduction pathways.

Basic Information

Product Name	Z-Phe-Val-Arg-Pna Hcl
CAS No.	69716-00-3
Molecular Formula	C34H43N9O7 • HCl
Molecular Weight	726.2 g/mol
Synonyms	Benzyloxycarbonyl-Phe-Val-Arg-p-nitroanilide hydrochloride; Z-Phe-Val-Arg-pNA • HCl; Chromogenic Substrate for Thrombin; N-Benzyloxycarbonyl-L-phenylalanyl-N-[(2S,3S)-1-[(2S)-5-guanidino-2-aminopentyl]-3-methyl-2-oxopentyl]-L-valinamide 4-nitroanilide hydrochloride; Thrombin Substrate I; Tos-Gly-Pro-Arg-pNA • HCl (common related substrate); S-2238 (common related substrate); Protease Substrate Z-F-V-R-pNA • HCl
EINECS	Contact for details

Quality Control

Our Z-Phe-Val-Arg-Pna Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific activity validation. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Optical Rotation	-25° to -30° (c=1 in DMF)
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Biological Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.