Description

Uridine Diphosphate n-Acetyl-D-Glucosamine, [Acetyl-1-14C] is a radiolabeled nucleotide sugar derivative essential for tracing and quantifying biochemical pathways in glycobiology and metabolic research. This compound serves as a critical tool for studying glycosyltransferase activity, glycoprotein biosynthesis, and carbohydrate metabolism with high sensitivity and specificity. It is primarily utilized by researchers in pharmaceutical development, academic biochemistry, and biotechnology sectors requiring precise isotopic labeling for advanced analytical applications.

Application

• Enzymatic Assay Substrate: Used as a radiolabeled donor substrate in assays for glycosyltransferases, such as GlcNAc transferases, to study enzyme kinetics and inhibition.
• Metabolic Pathway Tracing: Employed in pulse-chase experiments and metabolic flux analysis to track the incorporation of N-acetylglucosamine into glycoproteins, proteoglycans, and glycolipids.
• Glycobiology Research: Essential for in vitro studies of oligosaccharide and polysaccharide biosynthesis, helping to elucidate the mechanisms of glycan chain elongation and modification.
• Drug Discovery & Development: Supports high-throughput screening (HTS) campaigns for identifying novel inhibitors or modulators of glycosylation pathways relevant to cancer, inflammation, and infectious diseases.
• Biochemical Synthesis: Acts as a labeled precursor in the chemoenzymatic synthesis of complex, defined oligosaccharides and glycoconjugates for structural and functional studies.
• Cell Biology Studies: Used to investigate cell surface glycosylation changes, receptor-ligand interactions, and intracellular trafficking of glycoproteins in various cell lines.

Basic Information

Product Name	Uridine Diphosphate n-Acetyl-D-Glucosamine, [Acetyl-1-14C]
CAS No.	69616-95-1
Molecular Formula	C17H27N3O17P2 * (Labeled at Acetyl-1-14C)
Molecular Weight	~607.36 g/mol (unlabeled)
Synonyms	UDP-GlcNAc, [1-14C]Acetyl; UDP-N-acetyl-D-glucosamine, [acetyl-1-14C]; UDP-α-D-N-Acetylglucosamine, [acetyl-1-14C]; Uridine 5'-(trihydrogen diphosphate) P'→5'-ester with N-acetyl-α-D-glucosamine 1-phosphate, [acetyl-1-14C]; 14C-UDP-GlcNAc; Labeled UDP-N-acetylglucosamine; UDP-α-D-GlcNAc, [1-14C]Acetyl
EINECS	Contact for details

Quality Control

Every batch of our radiolabeled biochemicals is produced and tested under stringent quality control protocols to ensure high specific activity, radiochemical purity, and chemical stability. Analytical methods including HPLC with radiometric detection, NMR, and mass spectrometry are employed for comprehensive characterization. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting specific activity (mCi/mmol or GBq/mmol), radiochemical purity (typically >97%), chemical purity, and solvent composition. Our quality system supports research requiring reproducible and reliable data for publication and regulatory submissions.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at ≤ -20°C or as recommended on the specific COA. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. Store away from strong acids, bases, and oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white solid or frozen solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	50-60 mCi/mmol (1.85-2.22 GBq/mmol) (Typical)
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 95%
Solvent	Ethanol:Water mixture or as specified
Concentration	1.0 mCi/mL (37 MBq/mL) (Typical)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.