Description

26-Hydroxycholecalciferol is a key intermediate metabolite in the vitamin D3 (cholecalciferol) activation pathway, specifically formed in the liver. This compound is of significant interest for research into calcium homeostasis, bone metabolism, and the regulation of cell proliferation and differentiation. It serves as a critical reference standard and building block for pharmaceutical R&D, particularly in developing therapies for osteoporosis, renal disease, and certain cancers. High-purity 26-Hydroxycholecalciferol is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and advanced life science sectors.

Application

• Pharmaceutical Reference Standard: Used as a high-purity certified reference material (CRM) for analytical method development, validation, and quality control in drug manufacturing.
• Metabolic Pathway Research: Critical for in vitro and in vivo studies investigating the vitamin D metabolic cascade, enzymatic activity of CYP27A1, and related biological mechanisms.
• Drug Discovery & Development: Serves as a key intermediate or precursor in the synthesis of novel vitamin D analogs and metabolites with targeted therapeutic potential.
• Clinical Diagnostics: Employed in the calibration and standardization of LC-MS/MS and other assays for accurately quantifying vitamin D metabolites in human serum.
• Biochemical Research: Utilized in receptor binding studies, cell culture experiments, and investigations into the non-classical actions of vitamin D, such as immunomodulation.

Basic Information

Product Name	26-Hydroxycholecalciferol
CAS No.	69556-15-6
Molecular Formula	C₂₇H₄₄O₂
Molecular Weight	400.64 g/mol
Synonyms	26-Hydroxyvitamin D3; 26-Hydroxycholecalciferol; (3β,5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3,26-diol; 26(OH)D3; Vitamin D3 26-hydroxylase metabolite
EINECS	Contact for details

Quality Control

Our 26-Hydroxycholecalciferol is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. While produced for research use, our quality protocols align with the principles of current Good Manufacturing Practice (cGMP) where applicable. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. For short-term use, the material may be stored at 2-8°C. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solvent Residues (GC)	Meets ICH guidelines
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.